生物谷報(bào)道：四川大學(xué)研究人員近日在《公共科學(xué)圖書(shū)館·綜合》（PLoS ONE）發(fā)表研究論文,。
文章首次確認(rèn)可以使用D型氨基酸構(gòu)建新型自組裝短肽系統(tǒng),，由于D型短肽在體內(nèi)有更長(zhǎng)久的耐受性，因此,，這類(lèi)新型自組裝短肽將廣泛應(yīng)用于生物工程如細(xì)胞的三維培養(yǎng),、納米生物醫(yī)學(xué)工程如快速止血、修復(fù)神經(jīng)組織,，藥物工程如藥物控釋,、蛋白質(zhì)工程膜蛋白的穩(wěn)定與結(jié)晶等領(lǐng)域。該論文的長(zhǎng)遠(yuǎn)意義是對(duì)自組裝短肽的研究體系的完善和豐富,，對(duì)于開(kāi)啟設(shè)計(jì)符合戰(zhàn)略利益的新型納米生物材料,，占領(lǐng)基礎(chǔ)科學(xué)研究的科技前沿，具有重要的理論價(jià)值和實(shí)踐意義,。該文的潛在應(yīng)用是為自然災(zāi)害(地震災(zāi)害)和非自然災(zāi)害(戰(zhàn)爭(zhēng))中的創(chuàng)傷快速修復(fù)等帶來(lái)曙光,。（生物谷www.bioon.com）
生物谷推薦原始出處：
PLoS ONE，doi:10.1371/journal.pone.0002364,，Zhongli Luo,，Shuguang Zhang
Structural Dynamic of a Self-Assembling Peptide d-EAK16 Made of Only D-Amino Acids
Zhongli Luo, Xiaojun Zhao, Shuguang Zhang*
West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu, Sichuan, China
Abstract
We here report systematic study of structural dynamics of a 16-residue self-assembling peptide d-EAK16 made of only D-amino acids. We compare these results with its chiral counterpart L-form, l-EAK16. Circular dichroism was used to follow the structural dynamics under various temperature and pH conditions. At 25°C the d-EAK16 peptide displayed a typical beta-sheet spectrum. Upon increasing the temperature above 70°C, there was a spectrum shift as the 218 nm valley widens toward 210 nm. Above 80°C, the d-EAK16 peptide transformed into a typical alpha-helix CD spectrum without going through a detectable random-coil intermediate. When increasing the temperature from 4°C to 110°C then cooling back from 110°C to 4°C, there was a hysteresis: the secondary structure from beta-sheet to alpha-helix and then from alpha-helix to beta-sheet occurred. d-EAK16 formed an alpha-helical conformation at pH0.76 and pH12 but formed a beta-sheet at neutral pH. The effects of various pH conditions, ionic strength and denaturing agents were also noted. Since D-form peptides are resistant to natural enzyme degradation, such drastic structural changes may be exploited for fabricating molecular sensors to detect minute environmental changes. This provides insight into the behaviors of self-assembling peptides made of D-amino acids and points the way to designing new peptide materials for biomedical engineering and nanobiotechnology.
