低溫會(huì)導(dǎo)致水稻產(chǎn)量降低,。之前研究顯示,，低溫誘導(dǎo)的雄性不育常發(fā)生于水稻孕穗期。近期，來自日本北海農(nóng)業(yè)研究中心研究人員進(jìn)一步定位了抗寒基因Ctb1的位置,。研究者對(duì)Ctb1進(jìn)行了圖位克隆發(fā)現(xiàn),，Ctb1基因由兩部組成，他們分別編碼F-box蛋白和絲氨酸/蘇氨酸蛋白激酶,。
F-box蛋白常在水稻幼穗中表達(dá),，而絲氨酸/蘇氨酸蛋白激酶基因被證明在葉片和幼穗中表達(dá)。這兩種基因均從抗寒水稻品種中獲得,。研究者與其他抗寒基因的比較,，以及轉(zhuǎn)基因水稻子代第二代（T2)和第三代（T3）的后續(xù)分析。結(jié)果證明了F-box蛋白基因具有抗寒性,。F-box蛋白與Skp1基因（一種E3泛素連接酶基因）相互作用或可表明泛素蛋白酶通路和孕穗期抗寒機(jī)制有關(guān),。(生物谷Bioon.com)
生物谷推薦原文出處：
Plant Science doi:10.1016/j.plantsci.2010.04.004
Map-based cloning of the rice cold tolerance gene Ctb1
Koji Saito, a, , Yuriko Hayano-Saito a, Makoto Kuroki a and Yutaka Sato a
Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility. We previously mapped a quantitative trait locus for cold tolerance, Ctb1, to a 56-kb region containing 7 putative genes. In this study we further mapped Ctb1 to a 17-kb region containing two genes that encode an F-box protein and a ser/thr protein kinase. The F-box protein gene was preferentially expressed in young panicles, while the ser/thr protein kinase gene was expressed in leaves and young panicles. Both genes were cloned from a cold-tolerant variety, Norin-PL8, and introduced into a cold-sensitive variety, Hokkai241, and a cold-sensitive line, BT4-74-8. The cold tolerance of T2 and T3 progenies was assessed by measuring the degree of spikelet fertility in plants treated with cool water irrigation (19 °C, 25 cm) or cool air (12 °C, 4 days). The results indicated that the F-box protein gene confers cold tolerance. Cold tolerance is associated with greater anther length, and the transgenic plants had longer anthers than non-transformed controls. The F-box protein interacts with a subunit of the E3 ubiquitin ligase, Skp1, suggesting that an ubiquitin–proteasome pathway is involved in cold tolerance at the booting stage.
a National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Japan
