盡管RNA結構對其調控和功能很重要，但在實驗中已經了解的RNA結構相對很少,，而且也沒有對RNA結構進行高通過量測量的實驗方法,。相反，計算方法是全基因組應用的標準方法,。不過,，新技術已使人們能夠在更大規(guī)模上對RNA結構進行計算。
Kertesz等人利用一種深度測序方法來確定釀酒酵母整個轉錄組的結構,。他們獲得的結果為二級結構在翻譯中的作用提供了有趣的提示,，并且為研究這類結構何以能根據(jù)環(huán)境條件而改變搭建了舞臺。（生物谷Bioon.com）
生物谷推薦英文摘要：
Nature doi:10.1038/nature09322
Genome-wide measurement of RNA secondary structure in yeast
Michael Kertesz,Yue Wan,Elad Mazor,John L. Rinn,Robert C. Nutter,Howard Y. Chang Eran segal
The structures of RNA molecules are often important for their function and regulation1, 2, 3, 4, 5, 6, yet there are no experimental techniques for genome-scale measurement of RNA structure. Here we describe a novel strategy termed parallel analysis of RNA structure (PARS), which is based on deep sequencing fragments of RNAs that were treated with structure-specific enzymes, thus providing simultaneous in vitro profiling of the secondary structure of thousands of RNA species at single nucleotide resolution. We apply PARS to profile the secondary structure of the messenger RNAs (mRNAs) of the budding yeast Saccharomyces cerevisiae and obtain structural profiles for over 3,000 distinct transcripts. Analysis of these profiles reveals several RNA structural properties of yeast transcripts, including the existence of more secondary structure over coding regions compared with untranslated regions, a three-nucleotide periodicity of secondary structure across coding regions and an anti-correlation between the efficiency with which an mRNA is translated and the structure over its translation start site. PARS is readily applicable to other organisms and to profiling RNA structure in diverse conditions, thus enabling studies of the dynamics of secondary structure at a genomic scale.
