生物谷報(bào)道：最近,，研究人員在6月出版的《自然—細(xì)胞生物學(xué)》（Nature Cell Biology）期刊上報(bào)告說(shuō),，一種名為Caspase—14的蛋白質(zhì)與皮膚抗紫外線(xiàn)損害和水分流失的保護(hù)層的形成有關(guān)。
Caspase是指(ICE)/CED—3半胱氨酸蛋白酶家族, 目前有14個(gè)基因被證實(shí)屬于Caspase家族, 包括Caspase—1~Caspase—14, 其中人類(lèi)有11種: Caspase—1~Caspase—10,、Caspase—13,，而Caspase—11、Caspase—12具有種屬特異性, 僅存在于鼠中, Caspase—14是在鼠中被發(fā)現(xiàn)的,?？茖W(xué)家們?cè)缫阎溃鞍踪|(zhì)Caspase家族與細(xì)胞的程序化死亡和炎癥的發(fā)生有關(guān),，但他們一直沒(méi)有鑒別出Caspase—14的功能,。
利用Caspase—14被敲出的小鼠，Wim Declercq 和同事發(fā)現(xiàn),，Caspase—14負(fù)責(zé)在表皮的上層集中角蛋白和其他蛋白質(zhì),，形成角質(zhì)層。角質(zhì)層是由死亡細(xì)胞的殘骸形成的平坦層,，它們?cè)谄つw上形成一個(gè)保護(hù)層,。對(duì)前抗角蛋白微絲聚集蛋白形成抗角蛋白微絲聚集蛋白(Fillagrin)過(guò)程的控制，保證了上皮的完整性,。缺失Caspase—14的小鼠的皮膚出現(xiàn)了角質(zhì)層缺陷,，這些小鼠更容易出現(xiàn)失水，也更容易受到紫外線(xiàn)損傷,。
Figure 1. Histomorphological characterization of caspase-14-deficient epidermis.
(a) Measurement of WEHDamc and DEVDamc cleavage activity in epidermal extracts of wild-type and caspase-14 -/- mice. The error bars represent the mean s.d. of three mice. (b) Macroscopic observation of wild-type and caspase-14 -/- neonate mice. (c) Eosin–haematoxylin staining of skin sections of caspase-14 +/+ and caspase-14 -/- neonatal mice. (d) Scanning electron microscopy of epidermis of caspase-14 +/+ and caspase-14 -/- neonatal mice. (e) Transmission electron microscopy of caspase-14 +/+ and caspase-14 -/- neonate epidermis. Alveolar mottled keratohyalin F-granules are indicated by arrows. Neonates were 3.5–6.5 days old. (f) Confocal microscopy analysis with anti-filaggrin antibody on skin sections of 5.5-day-old mice. The scale bars represent 1 mm in b, 40 m in c, 100 m in d, 1 m in e and 10 m in f.
原文出處：
Caspase-14 protects against epidermal UVB photodamage and water loss
Geertrui Denecker, Esther Hoste, Barbara Gilbert, Tino Hochepied, Petra Ovaere, Saskia Lippens, Caroline Van den Broecke, Petra Van Damme, Katharina D'Herde, Jean-Pierre Hachem, Gaetan Borgonie, Richard B. Presland, Luc Schoonjans, Claude Libert, Joël Vandekerckhove, Kris Gevaert, Peter Vandenabeele, Wim Declercq
Nature Cell Biology 9, 666 - 674 (01 Jun 2007) Letters
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