將創(chuàng)建基于多模式成像的新組織分類方法用于對抗基底細(xì)胞癌,。人體中最多的惡性腫瘤?；准?xì)胞癌是人體中最多的惡性腫瘤,。這種疾病可表現(xiàn)出基于不同細(xì)胞生物學(xué)基礎(chǔ)的不同形態(tài)。
此外,，這種癌癥嵌在周圍的皮膚中,，肉眼無法看到起生長。因此,，在某些情況下,，這種癌癥的臨床檢測是一項挑戰(zhàn),。Jürgen Popp及其來自德國耶拿Institute of Photonic Technology及瑞典Lund University Hospital的同事希望創(chuàng)建一種基于多模式成像的非監(jiān)督組織分類法?；瘜W(xué)計量學(xué)將被用于區(qū)分基底細(xì)胞癌和非病變組織?，F(xiàn)在，他們提出一個案例研究,。這個案例研究結(jié)合應(yīng)用各種線性和非線性成像方式,，如熒光、拉曼和二次諧波產(chǎn)生顯微術(shù),，研究活體外解剖中基底細(xì)胞癌的形態(tài)化學(xué),。
這種新方法引出對樣本的形態(tài)化學(xué)探討。雖然結(jié)構(gòu)特征（即膠原蛋白結(jié)構(gòu)的存在）可通過SHG顯微術(shù)選擇性地記錄下來,，單分子和雙分子熒光揭示了樣本中天然熒光的空間分布,。最后，聯(lián)合應(yīng)用拉曼和CARS顯微光譜學(xué)允許樣本化學(xué)成分的互補(bǔ)特征,。
研究人員觀察到積極的基底細(xì)胞癌樣本中存在大量的儲存細(xì)胞或類脂物,。需進(jìn)行進(jìn)一步統(tǒng)計研究以證明這是否是一個重要標(biāo)志，如果是的話,，快速增長的惡性腫瘤擴(kuò)散血管中低營養(yǎng)是否可進(jìn)行補(bǔ)償,。不同技術(shù)的比較說明了在理解被調(diào)查組織的生物學(xué)上的互補(bǔ)性。（生物谷Bioon.com）
生物谷推薦英文摘要：
J.Biophotonics DOI: 10.1002/jbio.201000071
Multimodal imaging to study the morphochemistry of basal cell carcinoma
Nadine Vogler1,2, Tobias Meyer1, Denis Akimov1, Ines Latka1, Christoph Krafft1, Niels Bendsoe3, Katarina Svanberg4, Benjamin Dietzek1,2, Jürgen Popp2
Basal cell carcinoma is the most abundant malignant neoplasm in humans, the pathology of which is characterized by an abnormal proliferation of basal cells. Basal cell carcinoma can show a variety of different morphologies, which are based on different cellular biology. Furthermore, the carcinoma often grows invisibly to the eye imbedded in the surrounding skin. Therefore, in some cases its clinical detection is challenging. Thus, our work aims at establishing an unsupervised tissue classification method based on multimodal imaging and the application of chemometrics to discriminate basal cell carcinoma from non-diseased tissue. A case study applying multimodal imaging to ex-vivo sections of basal cell carcinoma is presented. In doing so, we apply a combination of various linear and non-linear imaging modalities, i.e. fluorescence, Raman and second-harmonic generation microscopy, to study the morphochemistry of basal cell carcinoma. The joint information content obtained by such multimodal approach in studying various aspects of the malignant tissue alterations associated with basal cell carcinoma is discussed.
