目前，乳腺癌病因?qū)W的研究主要集中在免疫及炎癥方面,。已知促炎癥因子IL-17A和IL-17F能夠介導(dǎo)炎癥和癌癥的發(fā)生,。為了評(píng)估在乳腺癌風(fēng)險(xiǎn)中IL-17A和IL-17F基因多態(tài)性的作用，來(lái)自中國(guó)哈爾濱醫(yī)科大學(xué)腫瘤預(yù)防及治療中心的龐達(dá)教授及其課題組在中國(guó)漢族女性中開展了一個(gè)乳腺癌病例對(duì)照研究,。研究發(fā)現(xiàn),，IL-17A中的單核苷酸多態(tài)性與乳腺癌發(fā)生風(fēng)險(xiǎn)有關(guān)。相關(guān)文章發(fā)表在3月26日的美國(guó)《公共科學(xué)圖書館·綜合》（PLoS One）上,。
研究人員通過(guò)在IL-17A中發(fā)現(xiàn)的3個(gè)單核苷酸多態(tài)性(SNPs)(rs2275913, rs3819025 and rs3748067)，以及在IL-17F中發(fā)現(xiàn)的5個(gè)SNPs(rs7771511, rs9382084, rs12203582, rs1266828和 rs763780),，來(lái)確定在491個(gè)女性乳腺癌患者及502個(gè)健康個(gè)體中的單體型,。通過(guò)SNaPshot技術(shù)研究者確定了基因型。而肺癌患者及健康個(gè)體的基因型分布差異則通過(guò)卡方檢驗(yàn)被分析,。
結(jié)果發(fā)現(xiàn),，對(duì)IL-17A中的rs2275913，AA基因型的頻率高于控制組(P = 0.0016),。臨床特征分析闡明了IL-17 SNPs與腫瘤蛋白53(P53),、黃體酮受體(PR)、人類表皮生長(zhǎng)因子受體2 (Her-2)以及三陰狀態(tài)（ER-/PR-/Her-2-）之間顯著的聯(lián)系,。
除此之外,，單體型分析表明了單體型Ars2275913Grs3819025Grs3748067的頻率，位于IL-17A連鎖不平衡模塊,，在患者中高于控制組(P = 0.0471),。
這項(xiàng)結(jié)果表明，IL-17A中的SNPs而不是IL-17F,，與乳腺癌發(fā)生風(fēng)險(xiǎn)有關(guān),。總之,，IL-17A及IL-17F的基因多態(tài)性可以提供有價(jià)值的信息,，來(lái)推測(cè)中國(guó)女性乳腺癌的預(yù)后。（生物谷Deepblue編譯）
doi: 10.1371/journal.pone.0034400
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Association Analysis of IL-17A and IL-17F Polymorphisms in Chinese Han Women with Breast Cancer
Lihong Wang, Yongdong Jiang, Youxue Zhang, Yuwen Wang, Sunhui Huang, Zhihua Wang, Baoling Tian, Yue Yang, Wei Jiang, Da Pang.
Research into the etiology of breast cancer has recently focused on the role of the immunity and inflammation. The proinflammatory cytokines IL-17A and IL-17F can mediate inflammation and cancer. To evaluate the influences of IL-17A and IL-17F gene polymorphisms on the risk of sporadic breast cancer, a case-control study was conducted in Chinese Han women.We genotyped three single-nucleotide polymorphisms (SNPs) in IL-17A (rs2275913, rs3819025 and rs3748067) and five SNPs in IL-17F (rs7771511, rs9382084, rs12203582, rs1266828 and rs763780) to determine the haplotypes in 491 women with breast cancer and 502 healthy individuals.The genotypes were determined using the SNaPshot technique. The differences in the genotypic distribution between breast cancer patients and healthy controls were analyzed with the Chi-square test for trends.For rs2275913 in IL-17A, the frequency of the AA genotype was higher in patients than controls (P = 0.0016). The clinical features analysis demonstrated significant associations between IL-17 SNPs and tumor protein 53 (P53), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and triple-negative (ER-/PR-/Her-2-) status.In addition, the haplotype analysis indicated that the frequency of the haplotype Ars2275913Grs3819025Grs3748067, located in the IL-17A linkage disequilibrium (LD) block, was higher in patients than in controls (P = 0.0471 after correction for multiple testing).Our results suggested that SNPs in IL-17A but not IL-17F were associated with the risk of breast cancer.Both IL-17A and IL-17F gene polymorphisms may provide valuable information for predicting the prognosis of breast cancer in Chinese women.<.
