microRNAs已經被發(fā)現(xiàn)是基因表達的重要調控因子，它們改變基因表達的作用與癌癥發(fā)生及腫瘤增殖密切相關,。因此，microRNAs能夠成為潛在的癌癥標記物及治療靶點,。
雖然已有的證據(jù)表明了microRNAs的異常表達與子宮內膜癌有關,，但是microRNAs對子宮內膜癌的預后意義有關的數(shù)據(jù)仍然有限。
近日,，美國紐約州立石溪大學的研究人員做了一項關于microRNAs的研究,。這項研究的目的在于，通過分析福爾馬林固定的石蠟包埋的組織,，來研究子宮內膜癌中有關的重要microRNAs的預后作用,。
實驗中,，使用試劑盒提取了來自48對正常的及子宮內膜癌標本的總RNAs。其中,，miR-26a,、let-7g、miR-21,、miR-181b,、miR-200c、miR-192,、miR-215,、miR-200c及miR-205的表達通過實時定量RT-PCR分析。差異表達的miRNAs通過免疫組化被定量,，數(shù)據(jù)分析則通過GraphPad Prism 5.0處理,。
結果發(fā)現(xiàn)，對比于正常組織,，miR-200c(P<0.0001)及miR-205(P<0.0001)的表達水平在子宮內膜瘤中被顯著提高,。
Kaplan-Meier生存分析顯示，高水平的miR-205表達與不良的病人存活率有關(hazard ratio,0.377;Logrank test,P=0.028),。此外,，在子宮內膜癌中發(fā)現(xiàn)，對比于正常組織,，靶向抑癌基因(PTEN)的miR-205的表達下降,。
這表明，在子宮內膜癌,，miR-205將是一個很有潛能的子宮內膜癌預后標記物。相關論文發(fā)表在3月13日的PLoS ONE,。（生物谷Deepblue編譯）
doi: 10.1371/journal.pone.0035158
PMC:
PMID:
Prognostic Significance of miR-205 in Endometrial Cancer
Mihriban Karaayvaz, Cecilia Zhang, Sharon Liang, Kenneth R. Shroyer, Jingfang Ju.
microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression.
Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets.Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer.The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach.The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis.Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.The expression levels of miR-200c (P<0.0001) and miR-205 (P<0.0001) were significantly increased in endometrial tumors compared to normal tissues.Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028).Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.
