中國(guó)科學(xué)院深圳先進(jìn)技術(shù)研究院生物醫(yī)藥與技術(shù)研究所（籌）蔡林濤研究員帶領(lǐng)的納米醫(yī)學(xué)研究小組近期在癌癥診斷技術(shù)研究上取得重要進(jìn)展,。
在癌癥診斷的研究中,，近紅外熒光納米檢測(cè)技術(shù)可以實(shí)現(xiàn)癌癥原位,、實(shí)時(shí)、靶向的無(wú)損監(jiān)測(cè)。吲哚菁綠（ICG）是一種具有近紅外特征吸收峰的三碳花菁染料,，是唯一一種被美國(guó)食品藥品監(jiān)督管理局（FDA）批準(zhǔn)的可用于臨床診斷的近紅外熒光染料,。但是ICG的穩(wěn)定性很差，在極性溶劑中會(huì)迅速聚集并分解,，且在光照環(huán)境下會(huì)加速分解，這給儲(chǔ)存和應(yīng)用帶來(lái)了困難,。同時(shí),，ICG在水溶液中的不穩(wěn)定性及在血漿中的快速清除率限制了它在熒光成像、目標(biāo)組織定位方面的應(yīng)用,。
蔡林濤課題組以ICG為熒光材料,，聚合物磷脂納米顆粒為載體，葉酸為靶向分子,，通過(guò)納米沉淀與自組裝的一步合成法成功開(kāi)發(fā)了一種熒光性能穩(wěn)定且對(duì)乳腺癌腫瘤細(xì)胞具有特異識(shí)別功能的近紅外熒光納米探針,。研究表明，通過(guò)裸鼠尾靜脈注射ICG納米探針能夠靶向識(shí)別腫瘤且在體內(nèi)的循環(huán)時(shí)間顯著長(zhǎng)于游離ICG,，表明該納米探針可用于腫瘤實(shí)時(shí)檢測(cè),，為腫瘤的早期診斷和藥物遞送系統(tǒng)的研究奠定了基礎(chǔ)。
相關(guān)成果發(fā)表在近期出版的Biomaterials上,。（生物谷Bioon.com）
doi:10.1016/j.biomaterials.2012.04.044
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Indocyanine green-loaded biodegradable tumor targeting nanoprobes for in vitro and in vivo imaging
Cuifang Zhenga, Mingbin Zhenga, b, Ping Gonga, Dongxue Jiaa, Pengfei Zhanga, Bihua Shia, Zonghai Shenga, Yifan Maa, Lintao Caia, ,
Indocyanine green (ICG) is a near-infrared (NIR) fluorescence dye for extensive biological application, but limited by its poor aqueous stability in vitro, concentration-dependent aggregation, rapid elimination from the body, and lack of target specificity. In this paper, to overcome these limitations, folate receptor-targeted, ICG dye-doped poly(d,l-lactide-co-glycolide) (PLGA) lipid nanoparticles (FA-ICG-PLGA-lipid NPs) were constructed by a single-step self-assemble and nanoprecipitation method. The prepared FA-ICG-PLGA-lipid NPs exhibited good biocompatibility, monodispersity, excellent NIR penetration ability, significant stability against photobleaching and long circulation time. The intracellular uptake experiment proved the targeting efficacy of the FA-ICG-PLGA-lipid NPs was more effective in folate receptor over-expressing MCF-7 cells than folate receptor negative A549 cells. Furthermore, the in vivo experiments showed the FA-ICG-PLGA-lipid NPs were specifically targeted to the tumor, and its circulation time was much longer than free ICG. These biocompatible and biodegradable NIR-NPs prove a potential application in tumor diagnosis and targeted imaging due to its high aqueous stability, excellent NIR optical properties and significantly targeting property in vivo
