姜黃素是從姜科、天南星科中的一些植物的根莖中提取的一種化學(xué)成分,，其中,，姜黃約含3%～6%，是植物界很稀少的具有二酮的色素,，為二酮類化合物,。
近來有報道姜黃素具有抗多種類型癌癥的作用,，但這種抗癌作用的分子機(jī)制尚不完全清楚。最近發(fā)表在Molecular Biology Reports雜志上的一項研究中,，研究人員評估分析了姜黃素對人類乳腺上皮細(xì)胞MCF-7細(xì)胞的作用,。細(xì)胞經(jīng)姜黃素處理后，MTT法檢測腫瘤細(xì)胞活力,。采用Transwell小室檢測細(xì)胞的侵襲能力,。NF-κB結(jié)合DNA的活性用跨AM NF-κB的ELISA試劑盒對核提取物進(jìn)行檢測。Western印跡檢測姜黃素對uPA表達(dá)的作用,。
研究結(jié)果表明姜黃素劑量依賴性地抑制MCF-7細(xì)胞增殖,。同時，姜黃素處理MCF-7細(xì)胞后,，其粘附和侵襲能力被大幅抑制,。姜黃素也顯著下降uPA的表達(dá)以及NF-κB的DNA結(jié)合活性。研究數(shù)據(jù)表明姜黃素通過抑制NF-κB的活化下調(diào)uPA的表達(dá),，拮抗MCF-7細(xì)胞的粘附和侵襲。研究人員希望姜黃素對乳腺癌的治療潛力值得進(jìn)一步研究,。（生物谷：Bioon.com）
doi:10.1007/s11033-011-1273-5
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Curcumin inhibits metastatic progression of breast cancer cell through suppression of urokinase-type plasminogen activator by NF-kappa B signaling pathways.
Zong H, Wang F, Fan QX, Wang LX.
Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), is extracted from the plant Curcuma longa. It was recently reported for its anticancer effect on several types of cancer cells in vitro however, the molecular mechanisms of this anticancer effect are not fully understood. In the present study, we evaluated the effects of curcumin on human mammary epithelial carcinoma MCF-7 cells. Cells were treated with curcumin and examined for cell viability by MTT assay. The cells invasion was demonstrated by transwell assay. The binding activity of NF-κB to DNA was examined in nuclear extracts using Trans-AM NF-κB ELISA kit. Western blot was performed to detect the effect of curcumin on the expression of uPA. Our results showed that curcumin dose-dependently inhibited (P < 0.05) the proliferation of MCF-7 cells. Meanwhile, the adhesion and invasion ability of MCF-7 cells were sharply inhibited when treated with different concentrations of curcumin. Curcumin also significantly decreased (P < 0.05) the expression of uPA and NF-κB DNA binding activity, respectively. It is concluded that curcumin inhibits the adhesion and invasion of MCF-7 cells through down-regulating the protein expression of uPA via of NF-κB activation. Accordingly, the therapeutic potential of curcumin for breast cancer deserves further study.
