8月2日,，刊登在國際著名雜志Journal of the National Cancer Institute上的一篇研究報告指出,，天然的植物化合物-苯乙基異硫氰酸酯（PEITC）可以抑制小鼠模型的乳房腫瘤的生長，小鼠的乳房腫瘤和人類的乳腺癌腫瘤非常相似,。食用植物可以作為化學(xué)的預(yù)防制劑，PEITC在小鼠的結(jié)腸癌、直腸癌等疾病中發(fā)揮著極強(qiáng)的化學(xué)預(yù)防因子的作用,。
為了研究PEITC在小鼠乳房腫瘤中的作用效率,，來自匹茲堡癌癥研究中心的研究者給予小鼠模型兩種飲食模式：一種節(jié)制飲食，另外一種為添加PEITC的飲食,，持續(xù)29周時間,。研究者隨后進(jìn)行了組織病理學(xué)的評估并且測定了小鼠機(jī)體中乳房腫瘤的尺寸、以及細(xì)胞增殖,、凋亡和新生血管發(fā)生的情況,。
研究者發(fā)現(xiàn)給予PEITC后，小鼠的乳腺腫瘤尺寸降低了56.3%,，盡管添加PEITC并不能完全抵御乳房的癌變,，但是飲食中添加PEITC的小鼠相比對照組，其機(jī)體表現(xiàn)出乳房癌的抑制現(xiàn)象,。
研究者最后指出了他們研究的局限性,，研究結(jié)果僅限于小鼠中，雖然和人類非常相似,，但是還是有一些不同,，而且添加PETIC和其促發(fā)癌細(xì)胞凋亡的機(jī)制關(guān)系尚不清楚。（生物谷Bioon.com）
編譯自：Plant-Based Compound Slows Breast Cancer in a Mouse Model
doi:10.1093/jnci/djs321
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Biomarkers of Phenethyl Isothiocyanate-Mediated Mammary Cancer Chemoprevention in a Clinically Relevant Mouse Model
Shivendra V Singh, Su-Hyeong Kim, Anuradha Sehrawat, Julie A Arlotti, Eun-Ryeong Hahm, Kozue Sakao, Jan H Beumer, Rachel C Jankowitz, Kumar Chandra-Kuntal, Joomin Lee, Anna A Powolny and Rajiv Dhir
Background Phenethyl isothiocyanate (PEITC) is a natural plant compound with chemopreventative potential against some cancers and the ability to induce apoptosis in breast cancer cells. Methods Female mouse mammary tumor virus–neu mice were fed a control AIN-76A diet (n = 35) or the same diet supplemented with 3 µmol PEITC/g diet (n = 33) for 29 weeks, at which time they were killed. Breast tissue sections were stained with hematoxylin and eosin for histopathological assessments, and incidence and size of macroscopic mammary tumors were assessed. Cell proliferation (Ki-67 staining), apoptosis (terminal deoxynucleotidyl transferase–mediated dUTP nick-labeling), and neoangiogenesis (CD31 staining) were determined in tumor sections. Plasma levels of transthyretin were measured in treated and control mice. Expression of proteins in mammary tumor sections was determined by immunohistochemistry. Proteomic profiling was performed by two-dimensional gel electrophoresis followed by mass spectrometry. All statistical tests were two-sided. Results Administration of PEITC for 29 weeks was associated with 53.13% decreased incidence of macroscopic mammary tumors (mean tumor incidence, PEITC-supplemented diet vs control diet, 18.75% vs 40.00%, difference = –21.25%, 95% confidence interval [CI] = –43.19% to 0.69%, P = .07) and with a 56.25% reduction in microscopic mammary carcinoma lesions greater than 2mm2 (mean incidence, PEITC-supplemented diet vs control diet, 18.75% vs 42.86%, difference = –24.11%, 95% CI = –46.35% to –1.86%, P = .04). PEITC-mediated mammary cancer growth inhibition was not because of suppression of human epidermal growth factor receptor-2 expression but was associated with reduced cellular proliferation and neoangiogenesis, increased apoptosis, and altered expression of several proteins, including decreased ATP synthase in the tumor and increased plasma levels of transthyretin. Conclusions PEITC inhibits the growth of mammary cancers in a mouse model with similarities to human breast cancer progression. ATP synthase and transthyretin appear to be novel biomarkers associated with PEITC exposure
