來自加州理工大學(xué)和加州大學(xué)圣地亞哥分校的研究者聯(lián)合研究蛋白晶體結(jié)構(gòu)，構(gòu)建了一個(gè)蛋白三維結(jié)構(gòu)數(shù)學(xué)模型,，破解了氨基酸鏈變蛋白三維結(jié)構(gòu)的秘密,。
研究人員使用加州理工大學(xué)研發(fā)的新技術(shù)獲得大量的實(shí)驗(yàn)數(shù)據(jù)構(gòu)建了一個(gè)蛋白折疊結(jié)構(gòu)模型,。
研究者使用一個(gè)能捕捉到皮秒圖像的攝影機(jī)來觀察蛋白結(jié)構(gòu)；鏈接蛋白與攝影機(jī)的是一個(gè)探針,，這個(gè)探針可捕捉激光的脈沖波激發(fā)出的熒光,，并將這一信息傳遞到模型的探針上。這些發(fā)光的信息能將鏈接蛋白的結(jié)構(gòu)秘密轉(zhuǎn)移到模型上,，這樣就可勾勒出蛋白的三維立體結(jié)構(gòu),。（生物谷Bioon.com）
生物谷推薦原始出處：
PNAS February 10, 2009 vol. 106 no. 6 1796-1801
Electrostatic effects on funneled landscapes and structural diversity in denatured protein ensembles
Patrick Weinkama, Ekaterina V. Pletnevab,1, Harry B. Grayb,2, Jay R. Winklerb,2 and Peter G. Wolynesa,2
aCenter for Theoretical Biological Physics and Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093; and
bBeckman Institute, California Institute of Technology, Pasadena, CA 91125
The denatured state of proteins is heterogeneous and susceptible to general hydrophobic and electrostatic forces, but to what extent does the funneled nature of protein energy landscapes play a role in the unfolded ensemble? We simulate the denatured ensemble of cytochrome c using a series of models. The models pinpoint the efficacy of incorporating energetic funnels toward the native state in contrast with models having no native structure-seeking tendency. These models also contain varying strengths of electrostatic effects and hydrophobic collapse. The simulations based on these models are compared with experimental distributions for the distances between a fluorescent donor and the heme acceptor that were extracted from time-resolved fluorescence energy transfer experiments on cytochrome c. Comparing simulations to detailed experimental data on several labeling sites allows us to quantify the dominant forces in denatured protein ensembles.
