9月2日,，國(guó)際著名期刊Nature在線發(fā)表了加拿大多倫多大學(xué)等研究人員的一篇題為Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae的研究論文,，文章首次描繪了膜蛋白相互作用組的全局拓?fù)鋱D,。
膜蛋白（membrane proteins ,，MPs）復(fù)合體在各種各樣的疾病中扮演著至關(guān)重要的角色,，是主要的藥物靶點(diǎn),。遺憾的是,，目前對(duì)模式生物體內(nèi)膜蛋白復(fù)合體的整體情況還沒(méi)有系統(tǒng)的描述,。
研究人員在沒(méi)有變性去垢劑存在的條件下親和純化了1590個(gè)釀酒酵母的脂錨定膜蛋白復(fù)合體,。這些共純化蛋白首先采用串聯(lián)質(zhì)譜的方法進(jìn)行鑒定，隨后用于繪制高可信度的膜蛋白相互作用物理圖,，其中包括1726對(duì)與各種細(xì)胞膜系統(tǒng)聯(lián)系的相互作用膜蛋白復(fù)合體和501個(gè)公認(rèn)的雜聚肽復(fù)合體 ,。
這些研究揭示了真核生物膜生物學(xué)潛在的物理聯(lián)系，描繪了膜蛋白相互作用組的全局拓?fù)鋱D,。(生物谷Bioon.com）
doi:10.1038/nature11354
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Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae
Mohan Babu,，James Vlasblom,Shuye Pu,Xinghua Guo,Chris Graham,Bj?rn D. M. Bean,Helen E. Burston,Franco J. Vizeacoumar,Jamie Snider,Sadhna Phanse, Vincent Fong,Yuen Yi C. Tam, Michael Davey,Olha Hnatshak,Navgeet Bajaj, Shamanta Chandran,Thanuja Punna,Constantine Christopolous,Victoria Wong, Analyn Yu,Gouqing Zhong,Joyce Li,Igor Stagljar, Elizabeth Conibear,Shoshana J. Wodak et al.
Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles and are prime drug targets in a variety of diseases1. Large-scale affinity purification studies of soluble-protein complexes have been accomplished for diverse model organisms, but no global characterization of MP-complex membership has been described so far. Here we report a complete survey of 1,590 putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae, which were affinity purified in the presence of non-denaturing detergents. The identities of the co-purifying proteins were determined by tandem mass spectrometry and subsequently used to derive a high-confidence physical interaction map encompassing 1,726 membrane protein-protein interactions and 501 putative heteromeric complexes associated with the various cellular membrane systems. Our analysis reveals unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome..
