中科院生態(tài)環(huán)境研究中心江桂斌研究組首次發(fā)現(xiàn)并確證了全氟碘烷的雌激素效應(yīng),，解釋了其構(gòu)效關(guān)系，為研究全氟類化合物的內(nèi)分泌干擾效應(yīng)提供了重要依據(jù),。相關(guān)論文近日發(fā)表在由美國國家環(huán)境健康研究所主辦的《環(huán)境與健康展望》（Environmental Health Perspectives）期刊上。
全氟碘烷類化合物是一類新型污染物,，關(guān)于其毒性效應(yīng)的研究目前屬于空白,。江桂斌等人通過細(xì)胞增殖實(shí)驗(yàn)、MVLN實(shí)驗(yàn)以及雌激素響應(yīng)基因在MCF-7乳腺癌細(xì)胞中的表達(dá),，研究了12種全氟碘烷的雌激素效應(yīng),。
研究發(fā)現(xiàn)，碘代全氟類化合物能夠誘導(dǎo)細(xì)胞增殖,，激活MVLN細(xì)胞中雌激素受體并誘導(dǎo)MCF-7細(xì)胞中TFF1和EGR3的表達(dá),，具有雌激素效應(yīng)。雌激素受體拮抗劑相關(guān)實(shí)驗(yàn)進(jìn)一步證實(shí)全氟碘烷類化合物激活雌激素受體的作用,。
同時(shí),，通過比較不同全氟類化合物和全氟碘烷的雌激素效應(yīng)，該研究發(fā)現(xiàn)全氟碘烷的雌激素效應(yīng)與全氟碳鏈的長度,、氟取代程度和碘元素取代相關(guān),。其結(jié)構(gòu)與傳統(tǒng)具有酚羥基結(jié)構(gòu)的雌激素類化合物具有明顯差異,。研究人員對(duì)全氟碘烷類化合物的雌激素效應(yīng)的機(jī)理進(jìn)行推測，認(rèn)為其羥基化代謝產(chǎn)物可能是激活雌激素受體的目標(biāo)分子,。（生物谷 Bioon.com）
doi:10.1289/ehp.1103773
PMC:
PMID:
The in Vitro Estrogenic Activities of Polyfluorinated Iodine Alkanes
Chang Wang, Thanh Wang, Wei Liu, Ting Ruan, Qunfang Zhou, Jiyan Liu, Aiqian Zhang, Bin Zhao, Guibin Jiang.
Background: Polyfluorinated iodine alkanes (PFIs) are important intermediates in the synthesis of organic fluoride products. Recently, PFIs have been detected in fluoropolymers as residual raw materials, as well as in the ambient environment.
Objectives: High production volumes and potential environmental releases of PFIs might become a concern, but the exposure risk and toxicity of these chemicals are still unclear. In this study, we investigated the potential estrogenic effects of PFIs.
Methods: We studied the estrogenic effects of fluorinated iodine alkanes (FIAs), fluorinated telomer iodides (FTIs), and fluorinated diiodine alkanes (FDIAs) using the E-screen and MVLN assays and the evaluation of estrogen-responsive genes in MCF-7 cells.
Results: FIAs have an iodine atom at one end of the perfluorinated carbon chain. 1-Iodoperfluorohexane (PFHxI) and 1-iodoperfluorooctane (PFOI) promoted the proliferation of MCF-7 cells, induced luciferase activity in MVLN cells, and up-regulated the expression of TFF1 and EGR3. In these assays, other FIAs gave negative responses. FDIAs have an iodine atom at each end of the perfluorinated carbon chain, and all the FDIAs showed estrogenic effects. The estrogenic potencies of FIAs and FDIAs correlate well with the carbon chain length of the chemicals. The optimum chain length for estrogenic effects is six carbons, and then eight and four carbons. All FTIs have a single iodine atom at the end of a partially fluorinated carbon chain. None of the FTIs showed estrogenic effects in the tests.
Conclusions: The estrogenic effects of PFIs are dependent on the structural features of iodine substitution and chain length. This research will be helpful in further understanding the estrogenic effects of perfluorinated compounds.
