昆蟲形態(tài)學是一門古老的學科,，一直在昆蟲分類學,、昆蟲系統(tǒng)學及昆蟲胚胎學等領域發(fā)揮著重要的作用。近年來,，隨著科技的發(fā)展,，新的技術和方法不斷地被引入該領域。計算機三維重建方法因其可以真實而直觀的反映動物體的空間形態(tài)結構,，近年來越來越多地被應用于動物形態(tài)學研究領域,。
由楊星科研究員領導的中科院動物所鞘翅目形態(tài)與進化研究組自2006年起一直致力于將基于顯微CT、激光共聚焦顯微鏡技術及組織切片技術的三維重建方法應用于昆蟲形態(tài)學研究,，并取得了重要進展,。主要包括應用顯微CT技術、計算機三維重建及系統(tǒng)發(fā)育分析等方法對葉甲亞科兩個屬榆葉甲屬和喜山葉甲屬的系統(tǒng)關系進行了深入探討，確立了二者的單系性,，并推測了上述兩個屬的起源及擴散方式，文章發(fā)表于昆蟲學領域核心期刊《系統(tǒng)昆蟲學》（Systematic Entomology）,；應用顯微切片技術,、激光共聚焦顯微技術及計算機三維重建方法，重構了鞘翅目寄生性甲蟲Lytta vesicatoria一齡幼蟲的全部肌肉,、神經系統(tǒng)及消化系統(tǒng),，探討了昆蟲微小化的結構特點，比較了與其他寄生性甲蟲及捻翅目的內外形態(tài)結構相似性,，文章發(fā)表于PLoS One,，目前尚有部分數(shù)據(jù)正在進一步整理之中。
此外,，在中國科學院重大裝備研制計劃資助下,，與中國科學院深圳先進技術研究院共同研制開發(fā)了應用于動物形態(tài)學研究的顯微CT設備，目前已落戶動物所,，等待驗收,。以上一系列成果不僅為進一步開展大規(guī)模的昆蟲乃至動物形態(tài)學研究工作及相關平臺建設奠定了堅實基礎，也為昆蟲形態(tài)學研究提供了全新的思路與方法,。(生物谷Bioon.com)
doi:10.1371/journal.pone.0052511
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The First Complete 3D Reconstruction of a Spanish Fly Primary Larva (Lytta vesicatoria, Meloidae, Coleoptera)
Si-Qin Ge, Benjamin Wipfler, Hans Pohl, Yi Hua, Adam Ślipiński, Xing-Ke Yang mail, Rolf Georg Beutel
The first detailed anatomical study of a primary larva of Meloidae is presented. Thereby techniques such as three-dimensional reconstructions, microtome sections, SEM (scanning electronic microscopy) and CLSM (confocal laser scanning microscopy) are applied. The structural features are discussed in the context of phylogeny, but also possible correlations with parasitism, phoresy and miniaturisation. The triungulin first instar larva is likely an apomorphy of Meloidae excl. Eleticinae and linked with a specialisation on acridoid eggs or larvae and provisions of bees. The campodeid body shape of Lytta and Meloinae is a groundplan feature of Meloidae, whereas a navicular body is an autapomorphy of the generally phoretic larvae of Nemognathinae. Head structures of Lytta and features of the postcephalic body are largely plesiomorphic. The musculature of the head is only moderately simplified while the one of the postcephalic body is well developed. Its thorax is largely characterised by plesiomorphies. The characteristics of the legs suggest phoretic habits, even though this does not apply to larvae of Lytta. It is conceivable that a phoretic behaviour is secondarily lost, together with some but not all morphological modifications related to it. Derived features of the abdomen of Meloidae are the complete loss of the fixed urogomphi (also missing in Rhipiphoridae and other related groups) and the presence of one or two conspicuous caudal bristles. Only few features of Lytta are shared with the parasitic larvae of Rhipiphoridae and Strepsiptera. These characteristics, which are possibly linked with specialised life habits, have obviously evolved independently. Miniaturisation effects are minimal in the larvae of Lytta.
