中國科學(xué)院昆明動(dòng)物研究所“百人計(jì)劃”賴仞研究員領(lǐng)導(dǎo)的研究團(tuán)隊(duì)通過蛋白質(zhì)組學(xué)結(jié)合藥理學(xué)研究手段從傳統(tǒng)抗血栓中藥材－牛虻唾液腺中識(shí)別了26種與抗血栓相關(guān)的活性蛋白質(zhì)或者多肽,。
牛虻在我國作為傳統(tǒng)的抗血栓藥物而長期使用，但其抗血栓的分子機(jī)制和物質(zhì)基礎(chǔ)還不清楚,，在去年該團(tuán)隊(duì)對(duì)牛虻抗血栓功能物質(zhì)進(jìn)行研究并取得了重要進(jìn)展的基礎(chǔ)上（Mol Cell Proteomics. 2008;7:582-90）,，該團(tuán)隊(duì)在該領(lǐng)域繼續(xù)深入研究并再次取得重要進(jìn)展，全面解析了牛虻作為傳統(tǒng)的抗血栓藥物的物質(zhì)基礎(chǔ)以及從其宿主成功獲取血液的分子機(jī)制,。該成果為開發(fā)新型安全的抗血栓藥物提供了豐富的分子資源,，同時(shí)也為生物防治牛虻提供了大量的作用靶標(biāo)。該研究結(jié)果已于2009年9月正式發(fā)表于國際著名學(xué)術(shù)刊物《分子與細(xì)胞蛋白質(zhì)組學(xué)》（Mol Cell Proteomics） （2009;8：2071－9）,。該研究工作得到了國際同行的高度評(píng)價(jià),，他們認(rèn)為“這項(xiàng)研究是采用蛋白質(zhì)組學(xué)手段研究非模式物種生物活性物質(zhì)的一個(gè)典范”。(生物谷Bioon.com)
生物谷推薦原始出處：
Molecular & Cellular Proteomics 8:2071-2079, 2009.
Anti-thrombosis Repertoire of Blood-feeding Horsefly Salivary Glands*,
Dongying Ma, Yipeng Wang, Hailong Yang, Jing Wu,, Shu An, Li Gao, Xueqing Xu, and Ren Lai,
From the Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China,
**Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China,
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China, and
Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
Blood-feeding arthropods rely heavily on the pharmacological properties of their saliva to get a blood meal and suppress immune reactions of hosts. Little information is available on antihemostatic substances in horsefly salivary glands although their saliva has been thought to contain wide range of physiologically active molecules. In traditional Eastern medicine, horseflies are used as anti-thrombosis material for hundreds of years. By proteomics coupling transcriptome analysis with pharmacological testing, several families of proteins or peptides, which exert mainly on anti-thrombosis functions, were identified and characterized from 60,000 pairs of salivary glands of the horsefly Tabanus yao Macquart (Diptera, Tabanidae). They are: (I) ten fibrin(ogen)olytic enzymes, which hydrolyze specially alpha chain of fibrin(ogen) and are the first family of fibrin(ogen)olytic enzymes purified and characterized from arthropods; (II) another fibrin(ogen)olytic enzyme, which hydrolyzes both alpha and beta chain of fibrin(ogen); (III) ten Arg-Gly-Asp-motif containing proteins acting as platelet aggregation inhibitors; (IV) five thrombin inhibitor peptides; (V) three vasodilator peptides; (VI) one apyrase acting as platelet aggregation inhibitor; (VII) one peroxidase with both platelet aggregation inhibitory and vasodilator activities. The first three families are belonging to antigen five proteins, which show obvious similarity with insect allergens. They are the first members of the antigen 5 family found in salivary glands of blood sucking arthropods to have anti-thromobosis function. The current results imply a possible evolution from allergens of blood-sucking insects to anti-thrombosis agents. The extreme diversity of horsefly anti-thrombosis components also reveals the anti-thrombosis molecular mechanisms of the traditional Eastern medicine insect material.
