生物谷報(bào)道：定量蛋白質(zhì)組學(xué)技術(shù)目前成為最為熱門的技術(shù)手段之一,。由荷蘭烏特勒支大學(xué)藥理科學(xué)系科學(xué)家Heck AJ等人剛剛在Mol Cell Proteomics雜志上發(fā)表的文章報(bào)道，采用定量蛋白質(zhì)組技術(shù)揭示了TATA結(jié)合蛋白（TBP）轉(zhuǎn)錄因子復(fù)合物的動(dòng)力學(xué)調(diào)節(jié)規(guī)律,。采用了親和純化和isotope蛋白質(zhì)標(biāo)簽（isotope labeling ）技術(shù)結(jié)合進(jìn)行研究,。這兩種技術(shù)結(jié)合能有效地,，且特異性檢測(cè)蛋白質(zhì)之間的相互作用，而且可以排除各類非特異性結(jié)合,，以減少假陽性,。同時(shí)還能檢測(cè)到那些瞬間結(jié)合起反應(yīng)的蛋白質(zhì)復(fù)合物，從而發(fā)現(xiàn)以往不能發(fā)現(xiàn)的蛋白質(zhì)之間相互作用，尤其是瞬間結(jié)合的反應(yīng),。在研究細(xì)胞周期的同步化中,，發(fā)現(xiàn)一種TAFs家族的BTAF1交換在其中起到關(guān)鍵性作用，它瞬間與其它蛋白質(zhì)結(jié)構(gòu),，從而調(diào)節(jié)了細(xì)胞的有絲分裂過程,。生物谷網(wǎng)專家認(rèn)為，這一新技術(shù)將在未來得到廣泛應(yīng)用,。蛋白質(zhì)之間相互作用,，有時(shí)是快速的，甚至瞬間的,，很難以檢測(cè),，傳統(tǒng)采用甲醛膠連等技術(shù)進(jìn)行檢測(cè)，仍然是屬于“碰運(yùn)氣”范圍,。這一新技術(shù)不僅更特異性,，而且更靈敏，極大拓展人類對(duì)蛋白質(zhì)間相互作用的認(rèn)識(shí),。
英文原文：
Affinity purification in combination with isotope labeling of proteins has proven to be a powerful method to discriminate specific from non-specific interactors. However, in the standard SILAC (Stable isotope labeling by amino acids in cell culture) approach dynamic components may easily be assigned as non-specific. We compared two affinity purification protocols, which in combination revealed information on the dynamics of protein complexes. We focused on the central component in eukaryotic transcription, the human TATA binding protein (TBP), which is involved in different complexes. All known TBP associated factors (TAFs) were detected as specific interactors. Interestingly, one of them, BTAF1, exchanged significantly in cell extracts during the affinity purification. The other TAFs did not display this behavior. Cell cycle synchronization showed that BTAF1 exchange was regulated during mitosis. The combination of the two affinity purification protocols allows a quantitative approach to identify transient components in any protein complex.
出處：
Quantitative proteomics reveals regulation of dynamic components within TATA-binding protein (TBP) transcription complexes.Mol Cell Proteomics. 2007 Dec 17
Mousson F, Kolkman A, Pijnappel WW, Timmers HT, Heck AJ.
Pharmmaceutical Sciences, Utrecht University, Utrecht 3584 CA.
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