日前,，在線出版的《自然—方法學(xué)》期刊上發(fā)表了一項(xiàng)研究成果表明，研究人員將庫存的胚胎干細(xì)胞和誘導(dǎo)性多能干細(xì)胞的蛋白質(zhì)組進(jìn)行比較,，發(fā)現(xiàn)兩種蛋白質(zhì)的表達(dá)有細(xì)微差異,，新發(fā)現(xiàn)有助于解釋這兩類細(xì)胞在分化為特定類型細(xì)胞上的能力差異。
與胚胎干細(xì)胞類似,，誘導(dǎo)性多能干細(xì)胞可分化為各種類型的人體細(xì)胞,，這種干細(xì)胞源自成人組織，它的使用避免了用胚胎干細(xì)胞進(jìn)行研究和醫(yī)學(xué)治療而出現(xiàn)的倫理問題,。然而,，問題是誘導(dǎo)性多能干細(xì)胞在生物學(xué)上是否完全等同于胚胎干細(xì)胞？
利用以高分辨率質(zhì)譜儀為基礎(chǔ)的蛋白質(zhì)組學(xué)分析方法,，Joshua Coon和同事分析了4個(gè)人類胚胎干細(xì)胞系和4個(gè)誘導(dǎo)性多能干細(xì)胞系的蛋白質(zhì),。根據(jù)可重復(fù)的分析結(jié)果，他們?cè)趦深惛杉?xì)胞的蛋白質(zhì)表達(dá)上發(fā)現(xiàn)了可重復(fù)的差異,。他們的結(jié)果顯示,，誘導(dǎo)性多能干細(xì)胞保持了它們所源自的已分化細(xì)胞的部分特征，這種特征可能指引它們發(fā)育成特定的細(xì)胞類型,。
作者將他們的數(shù)據(jù)放入一個(gè)可免費(fèi)進(jìn)入的新數(shù)據(jù)庫,。（生物谷 Bioon.com）
doi:10.1038/nmeth.1699
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Proteomic and phosphoproteomic comparison of human ES and iPS cells
Douglas H Phanstiel, Justin Brumbaugh, Craig D Wenger,Shulan Tian, Mitchell D Probasco, Derek J Bailey,Danielle L Swaney,Mark A Tervo, Jennifer M Bolin, Victor Ruotti, Ron Stewart, James A Thomson, & Joshua J Coon, ,
Combining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-scale protein quantification, we report deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate. This 24-sample comparison resulted in a very large set of identified proteins and phosphorylation sites in pluripotent cells. The statistical analysis afforded by our approach revealed subtle but reproducible differences in protein expression and protein phosphorylation between embryonic stem cells and induced pluripotent cells. Merging these results with RNA-seq analysis data, we found functionally related differences across each tier of regulation. We also introduce the Stem Cell–Omics Repository (SCOR), a resource to collate and display quantitative information across multiple planes of measurement, including mRNA, protein and post-translational modifications.
