Nuclear Membrane Proteins with Potential Disease Links Found by Subtractive Proteomics
Eric C. Schirmer, Laurence Florens,* Tinglu Guan, John R. Yates, III, Larry Gerace
To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested targeted to the NE, indicating that there are substantially more integral proteins of the NE than previously thought. Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies.
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
* Present address: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
To whom correspondence should be addressed. E-mail: [email protected] (L.G.); [email protected] (J.R.Y.)
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