封面圖片：發(fā)育中腎臟基因的藍(lán)圖通過一張熱圖表現(xiàn)，在熱圖的后面是一個發(fā)育中的腎臟的共聚焦圖像,，封面設(shè)計：Bruce Aronow,，Eric Brunskill，Todd Valerius）
腎臟的發(fā)育過程取決于大量基因的細(xì)胞種類特異性分化表達(dá),，盡管目前多個關(guān)鍵的基因和路徑都已經(jīng)得到了研究闡明,，但是科學(xué)家還從未得到對于單個細(xì)胞和解剖結(jié)構(gòu)的基因表達(dá)的整個基因組尺度的分析結(jié)果。在2008年11月11日出版的《發(fā)育細(xì)胞》（Developmental Cell）上,，來自美國的Brunskill等科學(xué)家得到了發(fā)育中腎臟的各個不同部分間基因表達(dá)模式的圖譜,。
研究小組在本期刊物的封面文章中詳細(xì)描述了他們得到的基因表達(dá)圖譜，在得到圖譜的過程中,，科學(xué)家利用激光捕獲顯微分離（laser capture microdissection）以及熒光激活細(xì)胞分揀術(shù)（fluorescence activated cell sorting）等方法得到了發(fā)育中的小鼠腎臟的各部分詳細(xì)數(shù)據(jù),，此外，研究人員還利用Affymetrix GeneChips芯片對腎臟樣本進(jìn)行了基因微陣列分析,。作者表示他們得到的結(jié)果與已知的表達(dá)模式符合,。
完成以上的圖譜能夠幫助科學(xué)家更好的研究基本的發(fā)育機(jī)制，例如間充質(zhì)細(xì)胞-上皮細(xì)胞轉(zhuǎn)變,，誘導(dǎo)信號,，形態(tài)發(fā)生等等。圖譜使得全面分析腎臟發(fā)生過程中基因表達(dá)狀態(tài)的發(fā)展變得可能,，除此之外,，作者表示它還有助于發(fā)現(xiàn)潛在的生長因子-受體相互作用。研究結(jié)果更好的揭示了腎臟發(fā)育的基因調(diào)節(jié)機(jī)制,。（生物谷Bioon.com）
生物谷推薦原始出處：
Developmental Cell,，Volume 15, Issue 5, 781-791, 11 November 2008，Eric W. Brunskill, S. Steven Potter
Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution
Eric W. Brunskill1,Bruce J. Aronow2,Kylie Georgas3,Bree Rumballe3,M. Todd Valerius4,Jeremy Aronow2,Vivek Kaimal2,Anil G. Jegga2,Sean Grimmond3,Andrew P. McMahon4,Larry T. Patterson5,Melissa H. Little3andS. Steven Potter1,,
1 Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
2 Division of Biomedical Informatics, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
3 Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
4 Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
5 Division of Nephrology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
SUMMARY
Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this couldprovide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional insitu hybridizations. This kidney atlas allowsa comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.
