栽培種落花生(Arachis hypogaea L.)是世界上第4大油料作物,,它主要生長(zhǎng)于熱帶、亞熱帶和暖溫帶地區(qū),。非洲和亞洲貧瘠地區(qū)的落花生生產(chǎn)面臨多種生物及非生物脅迫的挑戰(zhàn),。而分子標(biāo)記及遺傳圖譜是利用分子育種方法來(lái)抵制這些生物及非生物脅迫的必要前提。對(duì)于落花生而言,,盡管目前已經(jīng)開(kāi)發(fā)出了數(shù)百種分子標(biāo)記(例如微衛(wèi)星標(biāo)記或簡(jiǎn)單重復(fù)序列/SSR標(biāo)記),,并且在二倍體野生種或人工四倍體種作圖群體的基礎(chǔ)上得到了遺傳圖譜,但是目前還沒(méi)有有關(guān)栽培落花生的遺傳圖譜,。
國(guó)際半干旱熱帶作物研究所(ICRISAT)的一組科學(xué)家與巴西EMBRAPA/天主教大學(xué),、美國(guó)喬治亞大學(xué)和塔斯基吉大學(xué)的同事共同繪制出首例栽培種落花生SSR遺傳連鎖圖譜。這一圖譜包含135個(gè)SSR標(biāo)記,,22個(gè)連鎖群,。該研究小組已經(jīng)證明了這一遺傳圖譜在栽培種落花生性狀定位及豆類基因比較作圖中的應(yīng)用。(生物谷Bioon.com)
生物谷推薦原始出處:
Theor Appl Genet (2009) 118:729–739 DOI 10.1007/s00122-008-0933-x
The first SSR-based genetic linkage map for cultivated groundnut(Arachis hypogaea L.)
R. K. Varshney ?D. J. Bertioli ?M. C. Moretzsohn ?V. Vadez L. Krishnamurthy ?R. Aruna ?S. N. Nigam ?B. J. Moss ?K. Seetha K. Ravi ?G. He ?S. J. Knapp ?D. A. Hoisington
Abstract Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species.In case of peanut or groundnut (Arachis hypogaea L.),an amphidiploid (4X) species, not a single genetic map is,however, available based on a mapping population derived from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population. As a result,