韓國延世大學教授李仁錫（音譯）領導的研究小組首次揭開了全球4大糧食作物之一的水稻基因之間相互作用之謎。
研究小組稱：水稻有4萬多個基因,，他們已對其中的2萬個基因進行了分析,，并繪制完成揭示基因之間相互作用的“水稻基因網(wǎng)絡圖譜”。在這一過程中,，他們還發(fā)現(xiàn)對水稻免疫系統(tǒng)產(chǎn)生核心作用的3個基因,。
上述研究結(jié)果已刊載于權威科學雜志美國《國家科學院院刊》(PNAS)網(wǎng)絡版。(生物谷Bioon.com)
doi:10.1073/pnas.1110384108
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Genetic dissection of the biotic stress response using a genome-scale gene network for rice
Lee, Insuk; Seo, Young-Su; Coltrane, Dusica; Hwang, Sohyun; Oh, Taeyun; Marcotte, Edward M.; Ronald, Pamela C.
Rice is a staple food for one-half the world's population and a model for other monocotyledonous species. Thus, efficient approaches for identifying key genes controlling simple or complex traits in rice have important biological, agricultural, and economic consequences. Here, we report on the construction of RiceNet, an experimentally tested genome-scale gene network for a monocotyledonous species. Many different datasets, derived from five different organisms including plants, animals, yeast, and humans, were evaluated, and 24 of the most useful were integrated into a statistical framework that allowed for the prediction of functional linkages between pairs of genes. Genes could be linked to traits by using guilt-by-association, predicting gene attributes on the basis of network neighbors. We applied RiceNet to an important agronomic trait, the biotic stress response. Using network guilt-by-association followed by focused protein–protein interaction assays, we identified and validated, in planta, two positive regulators, LOC_Os01g70580 (now Regulator of XA21; ROX1) and LOC_Os02g21510 (ROX2), and one negative regulator, LOC_Os06g12530 (ROX3). These proteins control resistance mediated by rice XA21, a pattern recognition receptor. We also showed that RiceNet can accurately predict gene function in another major monocotyledonous crop species, maize. RiceNet thus enables the identification of genes regulating important crop traits, facilitating engineering of pathways critical to crop productivity.
