記者3月15日從中科院昆明動(dòng)物研究所獲悉,，該所梁斌課題組的博士張玉茹等研究人員，構(gòu)建了第一個(gè)秀麗線蟲(chóng)脂代謝基因數(shù)據(jù)庫(kù),，繪制了第一張秀麗線蟲(chóng)脂代謝途徑網(wǎng)絡(luò)圖,。該研究成果日前在線發(fā)表于《英國(guó)醫(yī)學(xué)委員會(huì)—基因組學(xué)》（BMC Genomics）。
據(jù)悉,，動(dòng)物模型對(duì)研究脂代謝調(diào)控和代謝性疾病是必不可少的,。在過(guò)去的10多年中，由于生活周期短,、低成本,、易培養(yǎng)和易操作等優(yōu)點(diǎn)，秀麗線蟲(chóng)成為一個(gè)研究脂代謝調(diào)控,、肥胖和肥胖相關(guān)代謝性疾病發(fā)病機(jī)理的良好模式動(dòng)物,。
然而，至今尚不清楚,，秀麗線蟲(chóng)基因組中有多少編碼脂代謝酶的基因和其參與的代謝途徑以及它們?cè)诠?、小鼠、大鼠和人中的保守性等?/p>
張玉茹等通過(guò)研究秀麗線蟲(chóng)基因組,，發(fā)現(xiàn)在秀麗線蟲(chóng)21000個(gè)左右的基因中,，至少有471個(gè)基因是編碼脂代謝酶的基因，參與16條不同脂類的代謝途徑,。同時(shí),，通過(guò)比較基因組學(xué)方法，他們進(jìn)一步從人,、小鼠、大鼠和果蠅基因組中分別發(fā)掘到581,、585,、563和428個(gè)脂代謝基因。
業(yè)內(nèi)專家表示，該研究第一次從基因組的角度分析了秀麗線蟲(chóng)脂代謝基因和代謝途徑,，為該領(lǐng)域未來(lái)的研究提供了大量資源,，并進(jìn)一步支持秀麗線蟲(chóng)是研究脂代謝和代謝性疾病的良好動(dòng)物模型。(生物谷Bioon.com)
DOI： 10.1186/1471-2164-14-164
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Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans
Yuru Zhang, Xiaoju Zou, Yihong Ding, Haizhen Wang, Xiaoyun Wu and Bin Liang
Animal models are indispensable to understand the lipid metabolism and lipid metabolicdiseases. Over the last decade, the nematode Caenorhabditis elegans has become a popularanimal model for exploring the regulation of lipid metabolism, obesity, and obese-relateddiseases. However, the genomic and functional conservation of lipid metabolism fromC. elegans to humans remains unknown. In the present study, we systematically analyzedgenes involved in lipid metabolism in the C. elegans genome using comparative genomics. Results We built a database containing 471 lipid genes from the C. elegans genome, and thenassigned most of lipid genes into 16 different lipid metabolic pathways that wereintegrated into a network. Over 70% of C. elegans lipid genes have human orthologs,with 237 of 471 C. elegans lipid genes being conserved in humans, mice, rats, andDrosophila, of which 71 genes are specifically related to human metabolic diseases.Moreover, RNA-mediated interference (RNAi) was used to disrupt the expression of 356of 471 lipid genes with available RNAi clones. We found that 21 genes strongly affectfat storage, development, reproduction, and other visible phenotypes, 6 of which havenot previously been implicated in the regulation of fat metabolism and other phenotypes. Conclusion sThis study provides the first systematic genomic insight into lipid metabolism inC. elegans, supporting the use of C. elegans as an increasingly prominent model inthe study of metabolic diseases.
