生物谷報道：另類拼接過程的存在,，能使一個基因產(chǎn)生多種蛋白?，F(xiàn)在，一種新型RNA圖被繪制出來,，該RNA圖可以反映短的RNA片段是怎樣影響另類拼接過程在大腦中被控制的方式的,。這一成果被發(fā)表于最新一期的《Nature》期刊上。
研究人員知道,，另類拼接過程為大腦中所發(fā)生的過程增添一層復雜性,，但它到底是怎樣被調控的（有時會失控而引起疾病）卻仍然不清楚,。該新型RNA圖將生物信息學,、生物化學和遺傳學方法結合起來確定一組規(guī)則，按照這些規(guī)則,，Nova基因調控小鼠體內神經(jīng)元特有的RNA結合蛋白的拼接增強子或沉寂子的活性,。這些結果有助于我們了解學習和記憶等大腦功能以及神經(jīng)疾病和癌癥生物學。
FIGURE 1. Definition of the Nova–RNA binding map.
a, A generic pre-mRNA showing the four regions that define the Nova–RNA binding map (the start and end of each region is labelled by a nucleotide distance to the splice site). Peaks demonstrate the positions of Nova-dependent splicing enhancers (red) or silencers (blue). b, A conserved YCAY cluster score (y axis) was calculated as described in Supplementary Methods. The x axis shows the nucleotide position of the centre of the sequence window. At each significant peak of YCAY cluster enrichment in Nova-regulated versus control pre-mRNAs, the P-value (two-tailed t-test, unequal variance) of YCAY clusters is shown. The error value of the control pre-mRNAs represents standard deviation of the mean values of 100 random groups of 20 control pre-mRNAs. c, YCAY cluster distribution in eight Nova-regulated pre-mRNAs that contain both NISE2 and NISE3 elements.
原文出處：
Nature Volume 444 Number 7119
An RNA map predicting Nova-dependent splicing regulation p580
Jernej Ule, Giovanni Stefani, Aldo Mele, Matteo Ruggiu, Xuning Wang, Bahar Taneri, Terry Gaasterland, Benjamin J. Blencowe and Robert B. Darnell
doi:10.1038/nature05304
Abstract | Full Text | PDF (1,073K) | Supplementary information
See also: Editor's summary
