熱激蛋白（HSPs）在生物體對環(huán)境適應(yīng)性上起到重要的作用,。農(nóng)業(yè)蟲鼠害綜合治理研究國家重點實驗室康樂研究組探討了熱激蛋白對斑潛蠅（Liriomyza sativae ) 冷耐受力和生長發(fā)育方面的作用機理。
科研人員克隆了3種小分子HSP（Hsp19.5,、Hsp20.8和Hsp21.7）和2種HSP60（TCP1α,，TCP1ζ），定量PCR分析表明,，3種小分子HSP均能被低溫所誘導(dǎo)表達,，而且Hsp20.8對低溫更加敏感，這意味著不同的小分子HSP可能響應(yīng)不同強度的低溫脅迫,。6種Hsp基因（Hsp19.5,、Hsp20.8、Hsp21.7,、TCP1α,、TCP1ζ和Hsp90、）的相對表達量在不同生長發(fā)育階段差異顯著,?？偟谋磉_趨勢是：小分子Hsp（Hsp19.5、Hsp20.8,、Hsp21.7）在蛹期表達量最高,，而大分子Hsp（TCP1α、TCP1ζ,、Hsp90）的表達量則隨著生長發(fā)育而遞增,。這意味著除響應(yīng)溫度脅迫外，熱激蛋白基因可能還在昆蟲的生長發(fā)育中起著一定的作用,。這一研究結(jié)果進一步增加了人們對熱激蛋白基因功能的了解,，并為研究昆蟲的生長發(fā)育和變態(tài)提供了新的思路。
相關(guān)研究發(fā)表在愛思唯爾期刊《昆蟲生理學(xué)雜志》（Journal of Insect Physiology）雜志上,。（生物谷Bioon.com）
生物谷推薦原始出處：
Journal of Insect Physiology Volume 55, Issue 3, March 2009
Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa
Li-Hua Huanga, Chen-Zhu Wanga and Le Kang, a,
aState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, B5 Datun Road, ChaoYang District, Beijing 100101, China
The vegetable leafminer, Liriomyza sativae has spread worldwide, causing serious loss of agricultural productivity. Heat shock proteins (HSPs) play important roles in the environmental adaptation of various organisms, and to explore the functions of HSPs in relation to cold tolerance and development in L. sativae, three full-length cDNAs of small heat shock protein genes (ls-hsp19.5, ls-hsp20.8 and ls-hsp21.7) and two partial cDNAs of tcp1 (the hsp60 homolog, ls-tcp1α and ls-tcp1ζ) were cloned, and their transcriptional expression during cold hardening and development was examined by real time quantitative PCR. The open reading frames (ORFs) of ls-hsp19.5, ls-hsp20.8 and ls-hsp21.7 are 516, 543 and 573 bp in length, encoding proteins with molecular weights (M.W.) of 19.5, 20.8 and 21.7 kDa, respectively. The 956 and 323 bp partial cDNAs were respectively sequenced from ls-tcp1α and ls-tcp1ζ. The expression profiles during cold hardening revealed that ls-tcp1s did not respond to cold stress. However, the three small hsps were significantly induced by cold, and ls-hsp20.8 was more cold-sensitive than the others. These results suggest that different shsp members may be responsible for cold stresses of different intensity. The expression of hsps during developmental processes revealed that the mRNA levels of small hsps reached a peak in the pupal stage, whereas the levels of large hsps, including two ls-tcp1s, hsp60 and hsp90 increased gradually with the developmental process. These results suggest that, in addition to a heat shock response, these HSPs may be involved in the development of L. sativae.
