10月1日，《實驗動物學(xué)雜志—生態(tài)遺傳學(xué)和生理學(xué)》發(fā)表了中國科學(xué)院水生生物研究所桂建芳研究員主持的實驗室關(guān)于紡錘素(spindlin)在銀鯽卵子發(fā)生,、卵母細胞成熟和受精過程中的動態(tài)分布和功能作用的研究論文，并在其特寫(FEATURES)欄目中重點評述了這一發(fā)現(xiàn),。

該雜志在特寫欄目評述說：“成熟卵母細胞貯備有驅(qū)動胚胎發(fā)育早期階段的母源效應(yīng)因子。此前的幾個研究曾建議紡錘素是一個這樣的因子,，孫等用銀鯽作為模型描述了其參與卵胚轉(zhuǎn)換的特征(pp461-473),。他們用抗體評價了銀鯽紡錘素的卵巢表達，觀察了該蛋白在初級卵母細胞核仁中的特異定位,。隨著卵母細胞成熟,，紡錘素標(biāo)記的核仁數(shù)由2-10個劇烈地增加到多達1000個；隨著卵黃發(fā)生開始,，紡錘素快速地散布到整個核質(zhì)中,；最后在成熟卵母細胞中與紡錘體纖維共定位。受精后,，紡錘素水平迅速下降,，到8細胞期檢測不到該蛋白。它似乎在早期胚胎發(fā)生中起了重要作用,，且注入有特異抗體的受精卵在紡錘體組裝,，染色體分離和卵裂中表現(xiàn)有致命的缺陷,。這些數(shù)據(jù)共同表明,，紡錘素是一個真正的母源效應(yīng)因子，在隨著受精即刻到來的事件中起了主要作用”,。

該研究得到國家基礎(chǔ)研究973計劃,、國家重點基金和國家大宗淡水魚類產(chǎn)業(yè)技術(shù)體系等項目的資助，主要由博士研究生孫敏等完成,。（生物谷Bioon.com）

生物谷推薦英文原文：

Journal of Experimental Zoology Part A: Ecological Genetics and Physiology DOI: 10.1002/jez.618

Dynamic distribution of spindlin in nucleoli, nucleoplasm and spindle from primary oocytes to mature eggs and its critical function for oocyte-to-embryo transition in gibel carp
Min Sun, Zhi Li, Jian-Fang Gui

Spindlin (Spin) was thought as a maternal-effect factor associated with meiotic spindle. Its role for the oocyte-to-embryo transition was suggested in mouse, but its direct evidence for the function had been not obtained in other vertebrates. In this study, we used the CagSpin-specific antibody to investigate CagSpin expression pattern and distribution during oogenesis of gibel carp (Carassius auratus gibelio). First, the oocyte-specific expression pattern and dynamic distribution was revealed in nucleoli, nucleoplasm, and spindle from primary oocytes to mature eggs by immunofluorescence localization. In primary oocytes and growth stage oocytes, CagSpin accumulates in nucleoli in increasing numbers along with the oocyte growth, and its disassembly occurs in vitellogenic oocytes, which implicates that CagSpin may be a major component of a large number of nucleoli in fish growth oocytes. Then, co-localization of CagSpin and β-tubulin was revealed in meiotic spindle of mature egg, indicating that CagSpin is one spindle-associated factor. Moreover, microinjection of CagSpin-specific antibody into the fertilized eggs blocked the first cleavage, and found that the CagSpin depletion resulted in spindle assembly disturbance. Thereby, our study provided the first direct evidence for the critical oocyte-to-embryo transition function of Spin in vertebrates, and confirmed that Spin is one important maternal-effect factor that participates in oocyte growth, oocyte maturation, and oocyte-to-embryo transition.