減數(shù)分裂過程中配對的同源染色體是如何相互識別在植物的研究工作相對發(fā)表文章較少,。雖然功能上是保守的,，但不同物種的同源染色體配對起始及機制可能不同。
韓方普實驗室長期從事植物減數(shù)分裂及著絲粒的表觀遺傳學研究,，該研究組以玉米減數(shù)分裂突變體及含有雙著絲粒染色體的植株為材料,，發(fā)現(xiàn)著絲粒配對先于端粒的bouquet并在同源染色體配對其重要作用。同源染色體配對起重要作用的是著絲粒的活性而不是著絲粒的特異序列,。
該研究結(jié)果于2013年10月18日在線發(fā)表于Plant Cell雜志上(doi:10.1105/tpc.113.117846 ),。韓方普實驗室博士生張晶為該論文的第一作者。相關(guān)研究得到科技部的資助,。(生物谷Bioon.com)
生物谷推薦的英文摘要
Plant Cell doi:10.1105/tpc.113.117846
Centromere Pairing in Early Meiotic Prophase Requires Active Centromeres and Precedes Installation of the Synaptonemal Complex in Maize
Jing Zhang, Wojciech P.Pawlowskic and Fangpu Han
Pairing of homologous chromosomes in meiosis is critical for their segregation to daughter cells. In most eukaryotes, clustering of telomeres precedes and facilitates chromosome pairing. In several species, centromeres also form pairwise associations, known as coupling, before the onset of pairing. We found that, in maize (Zea mays), centromere association begins at the leptotene stage and occurs earlier than the formation of the telomere bouquet. We established that centromere pairing requires centromere activity and the sole presence of centromeric repeats is not sufficient for pairing. In several species, homologs of the ZIP1 protein, which forms the central element of the synaptonemal complex in budding yeast (Saccharomyces cerevisiae), play essential roles in centromere coupling. However, we found that the maize ZIP1 homolog ZYP1 installs in the centromeric regions of chromosomes after centromeres form associations. Instead, we found that maize STRUCTURAL MAINTENANCE OF CHROMOSOMES6 homolog forms a central element of the synaptonemal complex, which is required for centromere associations. These data shed light on the poorly understood mechanism of centromere interactions and suggest that this mechanism may vary somewhat in different species.
