生物谷報道：四氫大麻酚（THC）是大麻中的一種活性成分,，海馬區(qū)是大腦中與記憶有重要關系的區(qū)域。研究人員在12月號的《自然—神經科學》期刊上說,，THC能干擾小鼠大腦海馬區(qū)域中神經細胞間的協同作用,，并認為這也許能解釋毒品為何會損害記憶,。

    Gyrgy Buzsaki和同事記錄了小鼠海馬區(qū)中多種神經細胞的活動。正常情況下,，這種區(qū)域中神經細胞會形成團簇,，以每秒4~10次的速度激發(fā)動能或神經脈沖,。但當研究人員將THC或相關的合成藥物注射進海馬區(qū)后，這種協同作用被破壞了,。

    這種藥物沒有改變動能產生的數量,，而是改變了它們同時發(fā)生的趨勢。在這種藥物的作用下,，小鼠的記憶水平低于正常水平,，研究人員由此推測，神經細胞的同時放電對海馬區(qū)功能的正常行使很重要,。

Figure 1. CB1 receptor activation decreases the power of the LFP oscillations.

 (a) Compressed wide-band recording of LFP (2 h). Insets, epochs with increasing time resolution reveal dynamic LFP patterns and single units. Scale bars (top-down), 20 min/0.5 mV, 150 ms/0.2 mV, 10 ms/0.2 mV. (b) Power spectra of respective segments in a. (c) Power spectrogram during the control condition and after increasing doses of CP55940. (d) Similar to c but the agonist application is preceded by injection of the CB1 inverse agonist SR141716A. Data from a single rat shown in a–d. (e,f) Group results, showing spectral differences from baseline. Thin lines, individual experiments; bold lines, mean for each drug condition.

原文出處：

Nature Neuroscience   December 2006, Volume 9 No 12

Cannabinoids reveal importance of spike timing coordination in hippocampal function pp1526 - 1533
David Robbe, Sean M Montgomery, Alexander Thome, Pavel E Rueda-Orozco, Bruce L McNaughton & György Buzsaki
Published online: 19 November 2006 | doi:10.1038/nn1801
Abstract | Full text | PDF (548K)  | Supplementary Information
See also: News and Views by Soltesz & Staley

作者簡介：

György Buzsaki

Welcome to the Buzsaki Lab
 

David Robbe, PhD
David Robbe's Home Page
Center for Molecular and Behavioral Neuroscience

Rutgers University

Current Position
Research Assistant Professor. Center for Molecular and Behavior Neuroscience, Rutgers, Newark, NJ, USA

Directed by Gyorgy Buzsaki, MD, PhD.  Fellowship from the Human Frontier Science Organization

Techniques mastered:  Tetrodes and silicone probes recording on head-restrained and freely moving rats. Patch-clamp recording in slice. Pharmacology. Behavior and programing of behavioral set-up (LabView). Analysis of spike train and local field potential (Matlab)

Education/Research Background
2003-2005 Post-doctoral Associate. Center for Molecular and Behavior Neuroscience, Rutgers, Newark, NJ, USA.

Techniques :  Tetrodes and silicone probes recording on head-restrained and freely moving rats. Pharmacology. Behavior, Analysis.

Directed by Gyorgy Buzsaki, MD, PhD. Granted by Human Frontier Science Organization and EMBO (first year)

1999-2002 Graduate student and PhD candidate. Subject: Synaptic Plasticity and Addiction. Specialty: Electrophysiology and pharmacology. Thesis Advisor: Dr. J. Bockaert. Under the scientific responsibility of Dr O. Manzoni. PhD obtained the 22nd of October 2002. Thesis grant from the French Ministry of Research and Technology

1998-1999 Diplomee dEtudes Approfondies (Post graduate professional diploma), "Neurobiology, Grade B, First.

University of Montpellier II

1997-1998 Maitrise (Master of Science ) de Biologie Cellulaire et Physiologie

University of Paris VI. Ecole Normale Superieure d'ULM Paris

1996-1997 Licence (Bachelor of Science) de Biologie Cellulaire et Physiologie

University of Paris VI. Ecole Normale Superieure d'ULM Paris

Professional Experiences

2001-2002 Teaching experiences at the University of Montpellier II

09/98-06/99 Centre National de Recherches Scientifiques UPR 9023

Training course: Molecular biology and pharmacology laboratory (Dr. L. Fagni). Subject: Regulation of mGlu5 membrane trafficking by Homer protein . Specialties : patch-clamp, immunofluorescence, neuronal transfection, primary cultures of cerebellum

07/97-08/98 Institut National de la Sante et de la Recherche Medicale U259

Training course: (Dr N. Abrous). Subject: Neurogenesis and PSA-NCAM in the adult rat hippocampus. Immunohistochemistry

Publications

Robbe,D., Montgomery,S.M., Thome,A.,Rueda-Orozsco,P.E., McNaughton,B.L., and Buzsaki,G.
Cannabinoids reveal importance of spike timing coordination in hippocampal function
Nature Neuroscience (2006), 9, 1526-33.

Mato,S., Robbe,D., Puente,N., Grandes,P., and Manzoni,O.J.
Presynaptic homeostatic plasticity rescues long-term depression after chronic Delta9-tetrahydrocannabinol exposure.
J. Neurosci. (2005), 25, 11619-11627.

Mato,S., Chevaleyre,V., Robbe,D., Pazos,A., Castillo,P.E., and Manzoni,O.J.
A single in-vivo exposure to delta 9THC blocks endocannabinoid-mediated synaptic plasticity.
Nat. Neurosci. (2004), 7, 585-586.

Robbe,D., Alonso,G., and Manzoni,O.J.
Exogenous and endogenous cannabinoids control synaptic transmission in mice nucleus accumbens.
Ann. N. Y. Acad. Sci. (2003), 1003, 212-225.

Ango,F., Robbe,D., Tu,J.C., Xiao,B., Worley,P.F., Pin,J.P., Bockaert,J., and Fagni,L.
Homer-dependent cell surface expression of metabotropic glutamate receptor type 5 in neurons.
Mol. Cell Neurosci. (2002), 20, 323-329.

Robbe,D., Alonso,G., Chaumont,S., Bockaert,J., and Manzoni,O.J.
Role of p/q-Ca2+ channels in metabotropic glutamate receptor 2/3-dependent presynaptic long-term depression at nucleus accumbens synapses.
J. Neurosci. (2002), 22, 4346-4356.

Robbe,D., Kopf,M., Remaury,A., Bockaert,J., and Manzoni,O.J.
Endogenous cannabinoids mediate long-term synaptic depression in the nucleus accumbens.
Proc. Natl. Acad. Sci. U. S. A (2002), 99, 8384-8388.

Robbe,D., Bockaert,J., and Manzoni,O.J.
Metabotropic glutamate receptor 2/3-dependent long-term depression in the nucleus accumbens is blocked in morphine withdrawn mice.
Eur. J. Neurosci. (2002), 16, 2231-2235.

Robbe,D., Alonso,G., Duchamp,F., Bockaert,J., and Manzoni,O.J.
Localization and mechanisms of action of cannabinoid receptors at the glutamatergic synapses of the mouse nucleus accumbens.
J. Neurosci. (2001), 21, 109-116.

Ango,F., bani-Torregrossa,S., Joly,C., Robbe,D., Michel,J.M., Pin,J.P., Bockaert,J., and Fagni,L.
A simple method to transfer plasmid DNA into neuronal primary cultures: functional expression of the mGlu5 receptor in cerebellar granule cells.
Neuropharmacology (1999), 38, 793-803.

Awards/Grants Received

EMBO long term fellowship in 2003

Human Frontier Long Term Fellowship 2004

Meetings

Robbe, D., Montgomery, S., and Buzsaki, G. (2005). Cannabinoids destroy cell assembly coordination in the hippocampus. SFN 35th Annual Meeting. Washington, DC, USA. November 12-16.

Robbe, D., Montgomery, S., and Buzsaki, G. (2005). Cannabinoid receptor activation disrupts cell assembly coordination in the hippocampus. Gordon Conferences : Cannabinoid Function in the CNS. Bates College, ME, USA. July 17-22.

Robbe, D. and Buzsaki, G. (2004). Cannabinoids and endocannabinoids on the hippocampal network of behaving rats. Human Frontier Annual Meeting. Hakone, Japan. May 14-18.

Robbe, D. and Buzsaki, G. (2004). Cannabinoids and endocannabinoids on the hippocampal network of behaving rats. Computational and System Neuroscience Meeting. Cold Spring Harbor Laboratory, NY, USA. March 24-28.

Robbe, D., Kopf, M., Remaury, A., Bockaert, J., and Manzoni, O. J. (2002). Endogenous cannabinoids mediate long-term synaptic plasticity in the nucleus accumbens. 3rd Forum of European Neuroscience. Paris, France. July 13-17.

Robbe, D., Bockaert, J., and Manzoni, O. J. (2002). Endogenous cannabinoids mediate long-term synaptic plasticity in the nucleus accumbens. Winter Conference on Neurochemistry. S�den, Austria. April 6-11.

Robbe, D., Bockaert, J., and Manzoni, O. J. (2001). L'activation des r�epteurs metabotropiques du glutamate du groupe II induit une d�ression �long terme �la synapse glutamatergique du noyau accumbens de souris. 5 Colloque de la Socit�des Neurosciences (french). Toulouse, France. May 28-31.

Robbe, D., Bockaert, J., and Manzoni, O. J. (2001). Control of synaptic plasticity by mGluR2/3 at the mouse nucleus accumbens synapses. SFN 31st Annual Meeting. San Diego, CA, USA. November 10-15.