生物谷報道：德國科學家8月20日宣布,，應(yīng)用特殊的調(diào)節(jié)蛋白,，可使大腦星形膠質(zhì)細胞形成為功能性神經(jīng)細胞,，從而在因受傷或疾病造成的受損腦細胞的替代治療方面取得重要進展。
由國家環(huán)境與健康研究中心干細胞研究所和慕尼黑大學Magdalena Götz教授領(lǐng)銜的研究小組發(fā)現(xiàn),，應(yīng)用單一調(diào)節(jié)蛋白即可使膠質(zhì)細胞形成新的功能性神經(jīng)細胞,。通過在定時顯微鏡下對這一轉(zhuǎn)變過程進行時實觀察，他們發(fā)現(xiàn)膠質(zhì)細胞在呈現(xiàn)一般的神經(jīng)細胞形狀之前,，需要幾天的程序調(diào)整時間,，而新生的神經(jīng)細胞還具有一般神經(jīng)細胞典型的電生理特性。
兩年前，該研究小組已經(jīng)證實膠質(zhì)細胞在發(fā)育過程中具有干細胞功能,，即意味著它們可分化為功能性神經(jīng)細胞,，但在發(fā)育后期膠質(zhì)細胞喪失了這一功能。為了能夠逆轉(zhuǎn)這一發(fā)育過程,，該研究小組對發(fā)育過程中膠質(zhì)細胞形成神經(jīng)細胞所必需的調(diào)節(jié)分子進行了研究,。他們將新生大腦中的這些調(diào)節(jié)蛋白加入膠質(zhì)細胞，證實調(diào)節(jié)蛋白確實可開啟神經(jīng)蛋白的表達,。
Figure 1. Ngn2 and Mash1 can reprogram astroglial cells to adopt a neuronal fate. a, Examples of astroglia transduced with a vector only encoding GFP (CMMP) 12 d after infection. Left, GFP immunoreactivity; right, TuJ1 immunoreactivity. b, Example of Mash1-transduced cell also positive for TUJ1 12 d after infection. c, Example of Ngn2-transduced cell double-positive for TUJ1 12 d after infection. d, Summary of three independent experiments in which the number of clones consisting of TuJ1-positive (neuronal), TuJ1-positive as well as -negative (mixed), and only TuJ1-negative (non-neuronal) cells is given as a percentage of all clones analyzed. Cells were fixed 12 d after infection and analyzed in three different experimental batches with >100 cells counted in each batch. For statistical analysis, a nonparametric ANOVA was used with a Dunn's post hoc analysis.
原文出處：
8 Aug 2007; 27 (32)
Benedikt Berninger, Marcos R. Costa, Ursula Koch, Timm Schroeder, Bernd Sutor, Benedikt Grothe, and Magdalena Götz
Functional Properties of Neurons Derived from In Vitro Reprogrammed Postnatal Astroglia
J. Neurosci. 2007 27: 8654-8664; doi:10.1523/JNEUROSCI.1615-07.2007 [Abstract] [Full Text] [PDF] [Supplemental Data]
作者簡介：
Dr. Benedikt Berninger
Ludwig Maximilians-University of Munich
Department of Physiological Genomics
Research Cooperation:
· FORNEUROCELL
(Position: Head of Research Division)
Projects:
· Entwicklung eines Differenzierungsverfahrens für humane Astrozyten in funktionelle Neurone
(Research Cooperation: FORNEUROCELL)
URL:
http://physinst.web.med.uni-muenchen.de/genomic.htm
