面神經(jīng)損傷的治療是臨床醫(yī)學(xué)面臨的一個重要挑戰(zhàn),。中國科學(xué)院遺傳發(fā)育所戴建武研究員再生醫(yī)學(xué)實驗室與南京鼓樓醫(yī)院合作,，制備了適合面神經(jīng)損傷修復(fù)的功能支架材料，并用動物模型驗證了此功能支架材料可以有效促進(jìn)面神經(jīng)損傷的修復(fù),。
在這項研究中，他們以天然支架材料膠原和層粘連蛋白為基礎(chǔ),，分別制備了神經(jīng)導(dǎo)管,，膠原 和層粘連蛋白有序支架，這兩種有序纖維可分別與具有膠原結(jié)合能力或?qū)诱尺B蛋白結(jié)合能力的生長因子CNTF和BDNF特異結(jié)合,，共同組合形成功能支架材料,；并建立了大鼠面神經(jīng)橫斷損傷模型，結(jié)果表明此功能支架材料具有良好的生物相容性和適宜的降解速度,，能夠誘導(dǎo)神經(jīng)纖維有序延伸以及軸突的再髓鞘化,，促進(jìn)面神經(jīng)傳導(dǎo)通路重建和運(yùn)動功能的恢復(fù)，顯示了良好的臨床應(yīng)用前景,。
這項工作受到中國科學(xué)院戰(zhàn)略性科技先導(dǎo)專項項目和國家自然基金委重點項目的資助,，研究結(jié)果發(fā)表于Biomaterials雜志。（生物谷Bioon.com）
doi: 10.1016/j.biomaterials.2012.10.031
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Induction of rat facial nerve regeneration by functional collagen scaffolds.
Cao J, Xiao Z, Jin W, Chen B, Meng D, Ding W, Han S, Hou X, Zhu T, Yuan B, Wang J, Liang W, Dai J.
Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L × LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L × LOCS, and then filled them into the collagen nerve conduit. They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery.
