《中國神經(jīng)再生研究（英文版）》雜志在2013年1月第3期出版的一項關(guān)于“Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor nanospheres”的研究發(fā)現(xiàn)，以下腰骶部背根神經(jīng)節(jié)的中央突受壓縊制備犬急性多重馬尾壓縊模型模擬臨床馬尾綜合征,，鞘內(nèi)注射可吸收緩釋聚(乳酸-羥基乙酸)-腦源性神經(jīng)營養(yǎng)因子納米微球后,，可提高急性多重馬尾壓縊模型犬背根神經(jīng)節(jié)感覺神經(jīng)元內(nèi)腦源性神經(jīng)營養(yǎng)因子的表達(dá)，減輕神經(jīng)元變性,。文章作者認(rèn)為,，鞘內(nèi)注射持續(xù)緩慢釋放的腦源性神經(jīng)營養(yǎng)因子納米粒，可促進(jìn)急性重度馬尾綜合征犬損傷神經(jīng)結(jié)構(gòu)及功能的修復(fù),。（生物谷Bioon.com）
DOI:10.3969/j.issn.1673-5374.2013.03.005
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Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles
Junming Tan1, Jiangang Shi2, Guodong Shi2, Yanling Liu3, Xiaohong Liu3, Chaoyang Wang1, Dechun Chen1, Shunming Xing1, Lianbing Shen1, Lianshun Jia2, Xiaojian Ye2, Hailong He2, Jiashun Li2
This study established a dog model of acute multiple cauda equina constriction by experimental constriction injury (48 hours) of the lumbosacral central processes in dorsal root ganglia neurons. The repair effect of intrathecal injection of brain-derived neurotrophic factor with 15 mg encapsulated biodegradable poly(lactide-co-glycolide) nanoparticles on this injury was then analyzed. Dorsal root ganglion cells (L7) of all experimental dogs were analyzed using hematoxylin-eosin staining and immunohistochemistry at 1, 2 and 4 weeks following model induction. Intrathecal injection of brain-derived neurotrophic factor can relieve degeneration and inflammation, and elevate the expression of brain-derived neurotrophic factor in sensory neurons of compressed dorsal root ganglion. Simultaneously, intrathecal injection of brain-derived neurotrophic factor obviously improved neurological function in the dog model of acute multiple cauda equina constriction. Results verified that sustained intraspinal delivery of brain-derived neurotrophic factor encapsulated in biodegradable nanoparticles promoted the repair of histomorphology and function of neurons within the dorsal root ganglia in dogs with acute and severe cauda equina syndrome.
