自發(fā)性分化是神經(jīng)干細胞的特性,，而之前的很多關于神經(jīng)干細胞定向分化的研究都沒有考慮到自發(fā)性分化對實驗結(jié)果的影響,，因此,，自發(fā)性分化成為研究神經(jīng)干細胞生物學特點及臨床應用必須面對的問題。

睫狀神經(jīng)營養(yǎng)因子是迄今惟一發(fā)現(xiàn)的可以促進成體大鼠海馬神經(jīng)祖細胞向膠質(zhì)和神經(jīng)元方向分化的神經(jīng)營養(yǎng)因子,，這與自發(fā)性分化的結(jié)果相似,。但是，睫狀神經(jīng)營養(yǎng)因子是否參與了神經(jīng)干細胞自發(fā)性分化過程,？

 睫狀神經(jīng)營養(yǎng)因子誘導后,，74％的大鼠海馬神經(jīng)祖細胞表達Tuj1

2013年2月第4期出版的《中國神經(jīng)再生研究（英文版）》（NEURAL REGENERATION RESEARCH）雜志中一項關于“Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors”的研究顯示，培養(yǎng)的海馬神經(jīng)祖細胞表達內(nèi)源性睫狀神經(jīng)營養(yǎng)因子,，睫狀神經(jīng)營養(yǎng)因子抗體能夠減緩海馬神經(jīng)祖細胞向神經(jīng)元和神經(jīng)膠質(zhì)細胞的分化,。因此作者認為，內(nèi)源性睫狀神經(jīng)營養(yǎng)因子介導海馬神經(jīng)祖細胞的自發(fā)分化,。(生物谷Bioon.com)

鏈接閱讀：

睫狀神經(jīng)營養(yǎng)因子及受體的研究進展

doi: 10.3969/j.issn.1673-5374.2013.04.002
PMC:
PMID:
Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors

Jun Ding, Zhili He, Juan Ruan, Ying Liu, Chengxin Gong, Shenggang Sun,  Honghui Chen.

Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.