根據(jù)2012年7月5日在線發(fā)表在Allergy期刊上的一項研究,，患有慢性鼻竇炎(chronic rhinosinusitis, CRS)的病人擁有濃度增加的游離輕鏈(free light chain, FLC)，特別是對同時患有慢性鼻竇炎和鼻息肉(CRS with nasal polyps, CRSwNP)的病人而言,，尤是如此,。
荷蘭烏得勒支大學(xué)研究員Tom Groot Kormelink和同事們分析了患有CRSwNP的病人和患有CRS但是未患上鼻息肉(CRS without nasal polyps, CRSsNP)的病人的鼻腔組織,、鼻分泌物和血清以便確定FLC的存在。與此同時,，研究人員也研究了不同治療方法對FLC表達的影響,。
研究人員發(fā)現(xiàn)患有CRS的病人在鼻分泌物和鼻粘膜組織勻漿液(nasal mucosal tissue homogenate)中FLC濃度增加，而在患有CRSwNP的病人組織中,，這種濃度增加是最為顯著的,。利用免疫組織化學(xué)方法證實鼻息肉組織中FLC濃度增加。利用甲強龍(methylprednisolone)或白細胞介素5(interleukin-5, IL-5)抗體治療患有CRSwNP的病人能夠?qū)е氯砘蚓植縁LC濃度下降,。
論文作者們寫到,，“在CRSwNP和CRSsNP疾病中，F(xiàn)LC的存在意味著它可能在調(diào)節(jié)鼻旁竇(paranasal cavity)局部免疫反應(yīng)發(fā)揮著作用,。再者,，利用IL-5抗體治療之后，局部FLC濃度下降可能意味著IL-5創(chuàng)造環(huán)境來促進FLC產(chǎn)生,。”(生物谷：Bioon.com)
本文編譯自Free light chains identified in chronic rhinosinusitis
doi: 10.1111/j.1398-9995.2012.02866.x
PMC:
PMID:
Local free light chain expression is increased in chronic rhinosinusitis with nasal polyps
T. Groot Kormelink1,†, L. Calus2, N. de Ruyck2,†, G. Holtappels2, C. Bachert2, F. A. Redegeld1,‡,*, P. Gevaert
Background Free light chain (FLC) concentrations are demonstrated to be increased in different inflammatory disorders and are proposed to mediate mast cell–dependent immune responses. A role for mast cells is suggested in chronic rhinosinusitis with nasal polyposis (CRSwNP), which is characterized by a local Th2 inflammatory response. However, clear mast cell–activating factors are not always apparent. In this study, the presence of FLCs in CRS patients with or without nasal polyps (CRSw/sNP) was investigated and the effect of different treatments on FLC expression was analyzed. Methods Nasal tissue, nasal secretion, and serum of control patients, patients with CRSwNP, and CRSsNP were analyzed for the presence of kappa and lambda FLC. The expression of FLCs in nasal polyp tissue was investigated using immunohistochemistry. In addition, FLC was measured in serum and nasal secretion of nasal polyp patients treated with methylprednisolone, doxycycline, anti-IL-5, or placebo. Results Free light chain concentrations were increased in nasal secretion and mucosal tissue homogenates in patients with chronic rhinosinusitis, and this effect was most prominent in CRSwNP patients. Immunohistochemical analysis confirmed the increased FLC concentrations in nasal polyp tissue. In CRSwNP patients, treatment with methylprednisolone or anti-IL-5 resulted in the reduction in systemic or local FLC concentrations, respectively. Conclusion The presence of FLC in CRSwNP and CRSsNP suggests a possible role in mediating the local immune reaction in the paranasal cavities. Furthermore, the decrease in local FLCs after treatment with anti-IL-5 presumes that IL-5 creates an environment that favors FLC production.
