2012年8月11日 訊 /生物谷BIOON/ --近日,，來自范德堡大學(xué)的研究者指出,，一種揭示人體針對(duì)感染產(chǎn)生的免疫效應(yīng)和識(shí)別涉及免疫反應(yīng)蛋白質(zhì)的3D成像技術(shù)可以為未來感染性疾病提供新的標(biāo)志物和新型的藥物開發(fā)思路。研究者結(jié)合了磁共振成像（MRI）和質(zhì)譜成像技術(shù)來顯現(xiàn)小鼠對(duì)于細(xì)菌感染所產(chǎn)生的炎癥免疫反應(yīng),。相關(guān)研究結(jié)果刊登在了國際雜志Cell Host & Microbe上。
研究者Skaar和其同事非常感興趣在動(dòng)物感染中的三維成像感染技術(shù)，通過這種技術(shù),，研究者可以是發(fā)現(xiàn)在特異的感染部位所產(chǎn)生的蛋白質(zhì),，MRI技術(shù)就提供了組織損傷的解剖學(xué)影像技術(shù)。質(zhì)譜成像技術(shù)可以直接測(cè)定組織中的蛋白質(zhì),、脂質(zhì)和其它代謝產(chǎn)物,。
這項(xiàng)新型技術(shù)允許研究者觀察到感染動(dòng)物的單一圖像，并且可以觀察到免疫系統(tǒng)的蛋白如何做出反應(yīng)以及感染的組織如何被定位,。研究者希望研究這些蛋白質(zhì)來開發(fā)出針對(duì)感染的新的標(biāo)志物,，這將會(huì)有效改善診斷學(xué)工具或者新的治療干預(yù)靶點(diǎn)。
這項(xiàng)技術(shù)對(duì)于科學(xué)家們研究感染性疾病,、癌癥以及自體免疫疾病的炎癥反應(yīng)非常重要,。盡管這種技術(shù)是非侵襲性的，其只適用于移除的病人組織,。相關(guān)研究由國立衛(wèi)生研究院提供支持,。（生物谷Bioon.com）
編譯自：New View of Body's Infection Response
doi:10.1016/j.chom.2012.04.018
PMC:
PMID:
Monitoring the Inflammatory Response to Infection through the Integration of MALDI IMS and MRI
Ahmed S. Attia, Kaitlin A. Schroeder, Erin H. Seeley, Kevin J. Wilson, Neal D. Hammer, Daniel C. Colvin, M. Lisa Manier, Joshua J. Nicklay, Kristie L. Rose, John C. Gore, Richard M. Caprioli, Eric P. Skaar
Systemic bacterial infection is characterized by a robust whole-organism inflammatory response. Analysis of the immune response to infection involves technologies that typically focus on single organ systems and lack spatial information. Additionally, the analysis of individual inflammatory proteins requires antibodies specific to the protein of interest, limiting the panel of proteins that can be analyzed. Herein we describe the application of matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) to mice systemically infected with Staphylococcus aureus to identify inflammatory protein masses that respond to infection throughout an entire infected animal. Integrating the resolution afforded by magnetic resonance imaging (MRI) with the sensitivity of MALDI IMS provides three-dimensional spatially resolved information regarding the distribution of innate immune proteins during systemic infection, allowing comparisons to in vivo structural information and soft-tissue contrast via MRI. Thus, integrating MALDI IMS with MRI provides a systems-biology approach to study inflammation during infection.
