據(jù)國外媒體報(bào)道,，研究人員首次證實(shí)一種新的篩選方法有潛力用于鑒定新的人類腫瘤病毒以及確定病毒感染導(dǎo)致了什么類型的癌癥,。
發(fā)現(xiàn)新病原過程中的一個(gè)主要的障礙就是鑒定篩選技術(shù)的不足,。因此,，迫切需要可靠的篩選方法,。
結(jié)膜癌（conjunctival carcinomas）一直都被懷疑是由一種直接的感染源引起的,。在這項(xiàng)研究中,，研究人員發(fā)明了一種叫做數(shù)字轉(zhuǎn)錄本減法（digital transcript subtraction, DTS）的過程,，即一個(gè)從數(shù)據(jù)庫減去已知人類序列、剩余非人類序列用于后續(xù)研究的系統(tǒng),。
用DTS系統(tǒng)分析241122個(gè)結(jié)膜癌細(xì)胞只發(fā)現(xiàn)了21個(gè)與之前來自人類數(shù)據(jù)庫的序列不一致的結(jié)果——這意味著該系統(tǒng)能夠用于篩選人類序列數(shù)據(jù)庫并且鑒定出最有可能的來源于病毒的序列,。
研究人員表示，DTS是一個(gè)簡(jiǎn)單的能發(fā)現(xiàn)新核苷酸的篩選方法,。它首次提供了一些病原類型的定量證據(jù),。研究人員將他們的研究成果發(fā)表在2007年10月的《病毒學(xué)雜志》（Journal of Virology）雜志上。
原始出處:
Journal of Virology, October 2007, p. 11332-11340, Vol. 81, No. 20
Human Transcriptome Subtraction by Using Short Sequence Tags To Search for Tumor Viruses in Conjunctival Carcinoma
Huichen Feng,1 Jennifer L. Taylor,1 Panayiotis V. Benos,2 Robert Newton,3 Keith Waddell,4 Sebastien B. Lucas,5 Yuan Chang,1* and Patrick S. Moore1*
Molecular Virology Program, University of Pittsburgh Cancer Institute,1 Departments of Computational Biology and Human Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15213,2 Epidemiology Unit, Radcliffe Infirmary, Cancer Research UK, Gibson Building, Oxford OX2 6HE, United Kingdom,3 Ruharo Eye Hospital, Mbarara,4 Uganda Virus Research Institute, Entebbe, Uganda5
Received 24 April 2007/ Accepted 30 July 2007
Digital transcript subtraction (DTS) was developed to subtract in silico known human sequences from expression library data sets, leaving candidate nonhuman sequences for further analysis. This approach requires precise discrimination between human and nonhuman cDNA sequences. Database comparisons show high likelihood that small viral sequences can be successfully distinguished from human sequences. DTS analysis of 9,026 20-bp tags from an expression library of BCBL-1 cells infected with Kaposi's sarcoma-associated herpesvirus (KSHV) resolved all but three candidate sequences. Two of these sequences belonged to KSHV transcripts, and the third belonged to an unannotated human expression sequence tag. Overall, 0.24% of transcripts from this cell line were of viral origin. DTS analysis of 241,122 expression tags from three squamous cell conjunctival carcinomas revealed that only 21 sequences did not align with sequences from human databases. All 21 candidates amplify human transcripts and have secondary evidence for being of human origin. This analysis shows that it is unlikely that distinguishable viral transcripts are present in conjunctival carcinomas at 20 transcripts per million or higher, which is the equivalent of approximately 4 transcripts per cell. DTS is a simple screening method to discover novel viral nucleic acids. It provides, for the first time, quantitative evidence against some classes of viral etiology when no viral transcripts are found, thereby reducing the uncertainty involved in new pathogen discovery.
* Corresponding author. Mailing address: Hillman Cancer Center, Molecular Virology Program, University of Pittsburgh Cancer Institute, 5117 Centre Ave., Ste. 1.8, Pittsburgh, PA 15213. Phone: (412) 623-7721. Fax: (412) 623-7715. E-mail for Patrick S. Moore: [email protected] . E-mail for Yuan Chang: [email protected]
