微生物學(xué)通報(bào) JAN 20，2008,，35(1)：45~49
(1. 南開(kāi)大學(xué)生命科學(xué)學(xué)院 天津 300071)
(2. 天津科技大學(xué)生物工程學(xué)院 天津 300222)
摘 要: 對(duì)以DL-2-氨基-∆2-噻唑啉-4-羧酸(DL-2-amino-∆2-thiazoline-4-carboxylic acid, DL-ATC)為底物原料, 經(jīng)微生物酶法催化合成L-半胱氨酸, 并進(jìn)一步氧化和分離純化產(chǎn)物L(fēng)-胱氨酸的生產(chǎn)工藝和條件進(jìn)行了研究,。建立了以惡臭假單胞菌TS1138 (Pseudomonas putida TS1138)全細(xì)胞為酶源, 反復(fù)多次催化底物合成L-半胱氨酸, 并以2.0%二甲基亞砜(DMSO)為氧化劑氧化生成L-胱氨酸, 進(jìn)而通過(guò)001×7型陽(yáng)離子交換樹(shù)脂純化胱氨酸的新工藝,。采用高效液相色譜法考察該方法L-胱氨酸的總收率可以達(dá)到78.55%, 純度為99.12%,。該方法簡(jiǎn)單高效, 解決了酶穩(wěn)定性差不能重復(fù)使用, 而固定化酶方法繁瑣成本高的問(wèn)題, 為我國(guó)L-半胱氨酸和L-胱氨酸的生產(chǎn)開(kāi)辟一條新途徑。
關(guān)鍵詞: 惡臭假單胞菌, DL-ATC, L-半胱氨酸, L-胱氨酸, 生產(chǎn)工藝
Study on L-cystine Conversion Technology by Pseudomonas putida TS1138
LIU Chun-Qin1 YU Yang-Sheng1 BAI Gang1* YANG Wen-Bo1
CHEN Ning2 HUAI Li-Hua2
(1. College of Life Sciences, Nankai University, Tianjin 300071)
(2. Bioengineering College, TianJin University of Science and Technology, Tianjin 300222)
Abstract: A technology of L-cystine production was studied in this paper, which included microbial enzy-matic conversion of DL-2-amino-∆2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine, subsequent oxi-dization of L-cysteine to L-cystine and its purification. The cells of Pseudomonas putida TS1138 could be repetitively used as the enzyme sources to convert the substrate DL-ATC to L-cysteine. After being oxidated by 2% dimethy-sulforide (DMSO), L-cystine could be harvested and further purified by the positive ion-exchange resin 001×7. High Performance Liquid Chromatography (HPLC) identified the purified L-cystine as having a total recovery of 78.55% and purity of 99.12%. This study demonstrated an efficient and convenient method for L-cystine production, which overcame the instability of enzymes, troublesome procedures and high cost of enzyme immobilization as contrasted to the traditional method. All in all, it pro-vides a new approach for industrial production of L-cystine as well as L-cysteine.
Keywords: Pseudomonas putida, DL-ATC, L-cysteine, L-cystine, Production process
全文鏈接：惡臭假單胞菌TS1138轉(zhuǎn)化生產(chǎn)L-胱氨酸的工藝研究
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