微生物學(xué)通報(bào) MAY 20, 2008, 35(5): 653~660
Microbiology 2008 by Institute of Microbiology, CAS
李 丹 黃 磊 李國強(qiáng) 李召雨 馬 挺 梁鳳來* 劉如林
(南開大學(xué)生命科學(xué)學(xué)院 天津市微生物功能基因組學(xué)重點(diǎn)實(shí)驗(yàn)室 分子微生物學(xué)與技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室 天津 300071)
摘 要: 從石油污染海域海底泥中篩選到的1株低溫石油烴降解菌, 經(jīng)鑒定為紅平紅球菌(Rhodococcus erythropolis), 命名為T7-2。該菌株能以十六烷為碳源代謝產(chǎn)生一種對柴油等烴類具良好乳化作用的生物乳化劑。研究表明, 該乳化劑主要由糖類,、脂類和蛋白質(zhì)組成, 其比例為55.43:31.24:12.65,。進(jìn)一步研究證實(shí), 該乳化劑糖類的單糖組成為甘露糖和鼠李糖; 脂類由十碳、十二碳,、十六碳及十八碳脂肪酸組成; 蛋白質(zhì)由16種氨基酸構(gòu)成。本文還對乳化劑的理化性質(zhì)進(jìn)行了分析, 發(fā)現(xiàn)它是一種性能穩(wěn)定,、乳化效率高,、適應(yīng)范圍較為廣泛的生物乳化劑, 對海洋污染生物修復(fù)及石油開采都具有潛在的應(yīng)用價(jià)值,。
關(guān)鍵詞: 生物乳化劑, 紅平紅球菌, 生物修復(fù), 烴降解菌
基金項(xiàng)目：國家自然科學(xué)基金資助項(xiàng)目(No. 50674058); 天津市應(yīng)用基礎(chǔ)及前沿技術(shù)研究計(jì)劃資助項(xiàng)目(No.08JCYBJC03900)
* 通訊作者：Tel: 022-23505967; : [email protected]
Study on the Bioemulsifier Produced by a Hydrocarbon-degrading Strain T7-2 and Its Physic-chemical Properties
LI Dan HUANG Lei LI Guo-Qiang LI Zhao-Yu MA Ting
LIANG Feng-Lai* LIU Ru-Lin
(College of Life Sciences, Nankai University, Tianjin Key Laboratory of Microbial Functional Genomics,
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071)
Abstract: A low-temperature hydrocarbon-degrading strain T7-2, isolated from sea-mud of Bohai polluted area and identified as Rhodococcus erythropolis, was found to produce an extracellular, nondialyzable emul-sifying agent (referred to as bioemulsifier) when grew with hexadecane as carbon source. The results showed that, this bioemulsifier which could remarkably emulsify hydrocarbons such as diesel oil, is consisted of three parts carbohydrates, lipids and proteins, the proportion of which was 55.43:31.24:12.65. The mono-saccharide compositions were identified as mannose and rhamnose; the lipid compositions included de-canoic acid, lauric acid, hexadecanoic acid and stearic acid, and the protein constituents were composed of sixteen amino acids. Besides, according to the study of the physic-chemical properties of the bioemulsifier, it possesses the obvious advantages of character stability, high function efficiency and wide adaptation range, therefore this bioemulsifier is believed to have extensive application values for bioremediation of marine oil pollution, petroleum exploitation and etc.
Keywords: Bioemulsifier, Rhodococcus erythropolis, Bioremediation, Hydrocarbon degrading bacteria
全文下載：烴降解菌株T7-2產(chǎn)生的生物乳化劑及其理化性質(zhì)研究
更多全文請查看鏈接：http://journals.im.ac.cn
聲明：本文由《微生物學(xué)通報(bào)》授權(quán)生物谷 www.bioon.com 網(wǎng)站發(fā)布，如需轉(zhuǎn)載請直接與中國科學(xué)院微生物研究所期刊聯(lián)合編輯部聯(lián)系并支付相應(yīng)費(fèi)用,，未經(jīng)授權(quán)不得轉(zhuǎn)載,，若轉(zhuǎn)載將付相應(yīng)的法律責(zé)任。
