微生物學(xué)通報 MAY 20, 2008, 35(5): 782~787
Microbiology 2008 by Institute of Microbiology, CAS
[email protected]
陶 劍1,2 鄭承綱1 張 斌1 郭文斌1 王淑芳2 宋存江1, 2*
(1. 南開大學(xué)生命科學(xué)學(xué)院分子微生物學(xué)與技術(shù)教育部重點實驗室 天津 300071)
(2. 南開大學(xué)生命科學(xué)學(xué)院生物活性材料教育部重點實驗室 天津 300071)
摘 要: 從土壤中分離到1株能夠合成中長鏈聚羥基脂肪酸酯(PHAMCL)的細菌, 經(jīng)分類鑒定命名為Pseudomonas mendocina NK-01,。對于該菌株在氮源限制的情況下, 利用葡萄糖合成的PHAMCL進行了結(jié)構(gòu)分析,。同時, 獲得了該菌株P(guān)HA合成酶基因phaC1, 利用大腸桿菌表達載體pBluescript SK-構(gòu)建了重組載體pBSphaC1, 并在E.coli JM109中成功表達。以所構(gòu)建的工程菌株進行了發(fā)酵合成。
關(guān)鍵詞: 中長鏈聚羥基脂肪酸酯, 門多薩假單胞菌, PHA合成酶
Synthesis of Medium-chain-length Polyhydroxyalkanoate (PHAMCL) by Pseudomonas mendocina NK-01
TAO Jian1,2 ZHENG Cheng-Gang1 ZHANG Bin1 GUO Wen-Bin1 WANG Shu-Fang2
SONG Cun-Jiang1,2*
(1. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071)
(2. The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071)
Abstract: A strain which can synthesize medium-chain-length polyhydroxyalkanoate (PHAMCL) was isolated from soil. It was named as Pseudomonas mendocina NK-01 through classification and identification. The PHAMCL was synthesized by P. mendocina NK-01 from glucose in the condition of limited nitrogen source. The structure of it was characterized. In order to construct a recombined plasmid pBSphaC1, an expressing plasmid of E.coli, after phaC1 gene encoding PHA synthase was cloned from Pseudomonas mendocina NK-01 by PCR amplification, it was linked into pBluescript SK-. PhaC1 gene was expressed successfully in E.coli JM109. Fermentation synthesis was also investigated by recombined E.coli.
Keywords: Medium-chain-length polyhydroxyalkanoate (PHAMCL), Pseudomonas mendocina, PHA syn-thase
全文下載：利用門多薩假單胞菌NK-01合成中長鏈聚羥基脂肪酸酯(PHAMCL)
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