微生物學(xué)報Acta Microbiologica Sinica    48(6)：745~749; 4 June 2008

基金項目: 國家科技支撐計劃(2006BAD06A01); 江蘇省高校重大基礎(chǔ)研究項目(05KJA23016)

*通訊作者,。Tel: +86-514-87991416; E-mail: [email protected] cn

作者簡介: 唐應(yīng)華(1981. ), 男, 湖南東安人, 博士研究生, 研究方向為動物性病毒致病分子機理研究。E-mail: [email protected]

唐應(yīng)華，吳培培,，彭大新,，龍進學(xué),，張評滸,，張文俊,，李彥芳,，汪文斌,，劉秀梵*

(揚州大學(xué)獸醫(yī)學(xué)院，農(nóng)業(yè)部畜禽傳染病學(xué)重點開放實驗室, 揚州 225009)

摘要：【目的】為了探討高致病性禽流感病毒對水禽致病性差異的分子致病機理,?！痉椒ā课覀儗囊傍喎蛛x到的H5N1 亞型禽流感病毒的生物學(xué)特性進行鑒定，其中A/mallard/Huadong/Y/2003(Y)是對麻鴨無致病性病毒,，而 A/mallard/Huadong/S/2005(S)是對麻鴨高致病性病毒,。利用反向遺傳技術(shù)構(gòu)建一系列單個和多個基因組合替換基因重排病毒，并驗證重排病毒在麻鴨上的致病力,?！窘Y(jié)果】研究表明，PB2, PB1, PA(3P), HA 單基因以及3P 基因組合替換的使S 病毒對麻鴨的毒力完全致弱,，但相應(yīng)的基因替換后僅使Y 病毒對麻鴨的毒力略有上升,。兩病毒的其它基因?qū)Χ玖τ绊戄^小?！窘Y(jié)論】H5N1 亞型禽流感病毒對麻鴨的致病力受多基因調(diào)控,，且這種調(diào)控作用在不同病毒骨架上的影響不一致，強毒受影響程度遠比弱毒的大,。

關(guān)鍵詞：H5N1,；致病力；反向遺傳,；麻鴨

中圖分類號：Q78 文獻標識碼：A 文章編號：0001-6209 (2008) 06-0745-05

Virulence Changes of H5N1 Avian Influenza Virus after Gene Reassortment

Yinghua Tang, Peipei Wu, Daxin Peng, Jinxue Long, Pinghu Zhang,

Wenjun Zhang, Yanfang Li, Wenbin Wang, Xiufan Liu*

(Key Laboratory of Animal Infectious Disease, Ministry of Agriculture, School of Veterinary Medicine Yangzhou University, Yangzhou 225009, China)

Abstract: [Objective] Recently H5N1 subtype avian influenza virus (AIV) is capable of mortality to aquatic bird. The molecular basis for the virulence of this virus is still poorly understood. [Methods] We characterized two H5N1 subtype viruses, A/mallard/Huadong/Y/2003 (Y) is nonpathogenic to mallard whereas A/mallard/Huadong/S/2005 (S) is highly pathogenic to mallard. Using reverse genetics, we constructed a series of single-gene or multiple-gene reassortants from these two viruses. [Results] Substitution of single-gene for PB2, PB1, PA (3P), HA and of combination for 3P gene resulted in complete attenuation of S virus in mallard. However, these corresponding substitutions only slightly increased virulence of Y virus in mallard. Other gene segments had little contribution to the virulence of both viruses. [Conclusion] These results indicate that the pathogenicity of H5N1 AIV to mallard was regulated by multiple gene segments, and these regulations had more sensitive effect on highly pathogenic virus backbone than on low pathogenic virus backbone.

Keywords: H5N1; pathogenicity; reverse genetics; mallard

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