新近出版的普通病毒學(xué)雜志《普通病毒學(xué)雜志》(Journal of General Virology，August 2008; Volume 89, Part 8)刊登了中科院水生所趙哲博士為第一作者的封面文章“Identification and characterization of a novel envelope protein in Rana grylio virus” （Journal of General Virology,，2008 89: 1866-1872）,，該項(xiàng)研究結(jié)果由張奇亞研究員負(fù)責(zé)的水生病毒學(xué)科組和桂建芳研究員負(fù)責(zé)的發(fā)育遺傳學(xué)學(xué)科組合作完成。
論文報(bào)道了一個(gè)新發(fā)現(xiàn)的蛙虹彩病毒RGV囊膜蛋白基因及功能特征,。該RGV 53R 基因的ORF長(zhǎng)1569bp,，編碼一個(gè)長(zhǎng)為522aa,、分子量大小約為54.7kDa的蛋白,，具有兩次跨膜結(jié)構(gòu)域,、N末端十四烷基化位點(diǎn)和兩個(gè)不變的半胱氨酸殘基這樣三個(gè)結(jié)構(gòu)特征。在病毒感染細(xì)胞后12h才開(kāi)始轉(zhuǎn)錄和表達(dá),，且能被AraC所抑制,，為RGV晚期表達(dá)基因。免疫熒光定位顯示其表達(dá)產(chǎn)物較早時(shí)候在細(xì)胞質(zhì)中呈顆粒狀分布,，定位于內(nèi)質(zhì)網(wǎng)上,，而晚期則定位于病毒加工廠?？贵w中和實(shí)驗(yàn)進(jìn)一步顯示53R抗體能明顯降低或延遲RGV的感染,，證實(shí)這是一個(gè)在病毒裝配和感染過(guò)程中發(fā)揮重要作用的虹彩病毒囊膜蛋白。Bioon
中科院水生所近兩年在水生病毒學(xué)領(lǐng)域取得一系列進(jìn)展,，有關(guān)研究結(jié)果已在國(guó)際系列病毒學(xué)及相關(guān)學(xué)科期刊上發(fā)表[Journal of Virology, 2008,，82: 6889-6901; Virology, 2008, 372:118-126; Journal of General Virology, 2008, 89:1866-1872; Virus Research, 2007,123:128–137; 2008, 132:86–96; Apoptosis, 2007, 12(9):1569-1577; Journal of Virological Methods.2008. 148: 205–210; Viral Immunology, 2006,19(4):637-645; 2007, 20(1): 150-159; Archives Virology, 2008, 153: 1143–1148]，并受到國(guó)際同行關(guān)注,。如揭示蛙虹彩病毒裝配過(guò)程,、以及發(fā)現(xiàn)RGV尿嘧啶脫氧核糖核苷三磷酸酶dUTPase基因在病毒復(fù)制中有重要作用的論文發(fā)表后，被英國(guó)學(xué)者在長(zhǎng)篇綜述中(Netherton et al, Adv Virus Res. 2007 ;70C :101-182)作為虹彩病毒細(xì)胞質(zhì)病毒加工廠的典型事例所引用,。（生物谷Bioon.com）
生物谷推薦原始出處：
Journal of General Virology,，89 (2008), 1866-1872，Jian-Fang Gui,，Qi-Ya Zhang
Identification and characterization of a novel envelope protein in Rana grylio virus
Zhe Zhao, Fei Ke, You-Hua Huang, Jiu-Gang Zhao, Jian-Fang Gui and Qi-Ya Zhang
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Wuhan 430072, PR China
Correspondence
Qi-Ya Zhang
[email protected]
Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iridoviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R–GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses.
