微生物學(xué)通報(bào) NOV 20, 2008, 35(11): 1760~1763
Microbiology 2008 by Institute of Microbiology, CAS
一株家養(yǎng)野豬源豬圓環(huán)病毒2型的分離與鑒定
王海燕 高 崧* 劉秀梵
(揚(yáng)州大學(xué) 農(nóng)業(yè)部畜禽傳染病學(xué)重點(diǎn)開放實(shí)驗(yàn)室 揚(yáng)州 225009)
摘 要: 應(yīng)用PCR方法從臨診疑似斷奶仔豬多系統(tǒng)衰竭綜合征(PMWS)家養(yǎng)野豬病例的淋巴結(jié)和脾臟中擴(kuò)增出預(yù)期長(zhǎng)度的豬圓環(huán)病毒2型(PCV2)的DNA片段, 在Dulac細(xì)胞中進(jìn)行分離和培養(yǎng), 擴(kuò)增全基因組序列后進(jìn)行同源性分析。結(jié)果顯示, 擴(kuò)增產(chǎn)物與家豬源參考毒株的序列同源性均在98%以上, 該病毒與家豬源病毒差異不大,。（生物谷Bioon.com）
關(guān)鍵詞: 家養(yǎng)野豬, 豬圓環(huán)病毒2 型, 分離, 鑒定
Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar
WANG Hai-Yan GAO Song* LIU Xiu-Fan
(Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009)
Abstract: The nucleic acid fragment of porcine circovirus type 2(PCV2)was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome (PMWS). The sample were then inoculated into the Dulac cells and passaged for 8 generation. The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence. The sequence of genome was compared with other 9 strains originated from piglets in the same area. The result showed that these strains were in high homology.
Keywords: Domesticated wild boar, Porcine circovirus type 2, Isolation, Identification
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