四川大公古鹽井中可培養(yǎng)中度嗜鹽菌的初步分析
馮 瑋1 向文良1,2 郭建華1 宋 鵬3 張 馳1 楊志榮1*
(1. 四川大學(xué)生命科學(xué)學(xué)院 生物資源與生態(tài)環(huán)境教育部重點(diǎn)實(shí)驗(yàn)室 成都 610064)
(2. 西華大學(xué)生物工程學(xué)院 成都 610039)
(3. 四川大學(xué)生命科學(xué)學(xué)院 成都 610064)
摘 要: 以四川自貢大公古鹽井鹽鹵為研究對象, 分離獲得112株中度嗜鹽菌,。經(jīng)形態(tài)特征,、生理生化特征和16S rRNA同源序列分析表明：112菌株分屬于細(xì)菌域的Planococcus、Halomonas,、Halobacillus,、Oceanobacillus和Virgibacillus屬, 與P. rifitiensis和H. venusta、H. trueperi,、H. blutaparonensis,、V. picturae在16S rRNA基因水平分別有100%和99%的高相似性。但菌株QW06,、QW12,、QW15和QW18分別與O. profundus、H. trueperi和H. blutaparonensis在菌落形態(tài),、革蘭氏染色,、產(chǎn)酸、明膠水解和淀粉水解等表型特征上有明顯的差異,。同時(shí), 16S-23S rRNA ISR-PCR基因指紋圖譜也表明QW06,、QW12、QW15和QW18不同于參考菌株O. profundus KCCM 42318和H. trueperi DSM 1040T,、H. blutaparonensis ATCC BAA 1217T, 說明QW06,、QW12、QW15和QW18分類地位有待進(jìn)一步確定,。實(shí)驗(yàn)結(jié)果不僅揭示了大公古鹽井中可培養(yǎng)中度嗜鹽菌的多樣性和系統(tǒng)發(fā)育關(guān)系, 而且也表明了16S rRNA高度相似菌的16S-23S rRNA ISR在進(jìn)化的過程中發(fā)生了某些突變,。（生物谷Bioon.com）
關(guān)鍵詞: 大公古鹽井, 中度嗜鹽菌, 表型分析, 16S rRNA系統(tǒng)發(fā)育, 16S-23S rRNA ISR特性分析
A Primary Investigation on the Culture-dependent Moderately Halophilic Bacteria Colonizing in
Dagong Ancient Brine Well, Sichuan
FENG Wei1 XIANG Wen-Liang1, 2 GUO Jian-Hua1 SONG Peng3
ZHANG Chi1 YANG Zhi-Rong1*
(1. College of Life Sciences, Key Laboratory of Bio-resource and Bio-control, Sichuan University, Chengdu 610064)
(2. College of Bioengineering, Xihua University, Chengdu 610039)
(3. College of Life Sciences, Sichuan University, Chengdu 610064)
Abstract: 112 aerobic moderate halophilic bacteria were isolated from Daong Ancient Brine Well located in Zigong city, Sichuan Province, China. And they were subjected to the analysis of phenotype, physiology, 16S rRNA sequence. Furthermore, the intra specific phylogeny of closely related strains was also screened by PCR fragment length polymorphism of 16S-23S ribosomal RNA intergenic spacer regions (ISR). The re-sult showed that the halophilic isolates found in current study were closely related to the following genera: Planococcus, Halomonas, Halobacillus, Oceanobacillus and Virgibacillus, a lineage of the domain Bacteria. 16S rRNA analysis revealed that their sequences sharing 100% and 99% similarity were obtained from the GenBank database for P. rifitiensis, H. venusta, H. trueperi, H.blutaparonensis, O. profundus and V. pciturae. However, isolates QW06、QW12,、QW15 and QW18 exhibited differences from their corresponding reference strains to some degree, including colony pigmentation, gram staining, acid production and hydrolysis of gelatin, casein and starch. The ISR analysis disclosed variation of banding pattern in these isolates related to Oceanobacillus and Halobacillus which was observed with phenotypic and physiological characterizations as well. It was clear that these halophiles have adapted to the special man-made hypersaline environment by the basic physiological evolution during phylogenesis. So they could be determined according to further polyphasic taxonomy data. The current primary result also indicated the diversity of species of culture-dependent moderately halophilic bacteria colonizing in Dagong Ancient Brine Well as well as phenotypic and genotypic and phylogenetic diversity.
Keywords: Dagong ancient brine well, Moderately halophilic bacteria, Phenotype, 16S rRNA, 16S-23S rRNA ISR
生物谷推薦全文下載：四川大公古鹽井中可培養(yǎng)中度嗜鹽菌的初步分析
附表一：分離菌株及參考菌株的形態(tài)和部分生化特征
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