生物工程學(xué)報(bào) 25 August 2009, 25(8):1144～1150

百草枯對(duì)木質(zhì)素降解菌產(chǎn)酶及其生物化學(xué)變化的影響

趙蕓晨1,2, 李建龍1, 陳育如3, 黃海霞1, 余醉1

1 南京大學(xué)生命科學(xué)院 國(guó)家醫(yī)藥重點(diǎn)實(shí)驗(yàn)室, 南京 210093

2 河西學(xué)院農(nóng)學(xué)系, 張掖 734000

3 南京師范大學(xué)生命科學(xué)學(xué)院, 南京 210046

摘  要: 為研究外源活性氧對(duì)木質(zhì)素降解菌的影響, 本實(shí)驗(yàn)對(duì)外源百草枯誘導(dǎo)下的雜色云芝(Coriolus versicolor)產(chǎn)酶及其生物化學(xué)過(guò)程進(jìn)行了研究。將一定濃度的百草枯加入培養(yǎng)7 d的雜色云芝菌培養(yǎng)液中, 連續(xù)培養(yǎng)148 h, 測(cè)定其胞外木質(zhì)素降解酶,、胞內(nèi)抗氧化酶的活性及生物化學(xué)參數(shù)的變化,。與對(duì)照相比, 30  mol/L的百草枯能夠顯著促進(jìn)雜色云芝錳依賴過(guò)氧化物酶(MnP),、木質(zhì)素過(guò)氧化物酶(LiP)和漆酶(Lac)的活性, 3種酶活性分別提高了1.3,、7和2.5倍; 在連續(xù)培養(yǎng)的前48 h, 30  mol/L的百草枯促進(jìn)了胞內(nèi)超氧化物歧化酶(SOD)、過(guò)氧化氫酶(CAT)的活性,。百草枯對(duì)于胞外木質(zhì)素降解酶活性的促進(jìn)作用比對(duì)胞內(nèi)抗氧化酶活性的促進(jìn)作用明顯,。百草枯的加入促進(jìn)了胞外酚類化合物與甲醛的濃度的增加, 而丙二醛的濃度在培養(yǎng)的前24 h內(nèi)增加, 隨后下降。結(jié)果表明, 百草枯的加入對(duì)白腐菌產(chǎn)生了氧化脅迫, 但菌株的抗氧化系統(tǒng)能夠有效地進(jìn)行氧化劑的清除, 從而阻止氧化劑對(duì)機(jī)體的氧化傷害,。百草枯作為外源氧化脅迫劑, 可以增加木質(zhì)素降解酶活性, 有利于木質(zhì)素的降解,。

關(guān)鍵詞: 雜色云芝, 百草枯, 氧化脅迫, 木質(zhì)素降解酶, 甲醛

Impact of exogenous paraquat on enzyme exudation and biochemical changes of lignin degradation fungi

Yunchen Zhao1,2, Jianlong Li1, Yuru Chen3, Haixia Huang1, and Zui Yu1

1 State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China

2 Department of Agronomy, Hexi University, Zhangye 734000, China

3 School of Life Science, Nanjing Normal University, Nanjing 210046, China

Abstract: To study the effect of exogenous oxygen, we added water solution of paraquat to 7 d cultures of Coriolus versicolor for the next 148 h. Enzyme exudation and biochemiscal process were investigated on the addition of paraquat. We found that compared with the control (without paraquat), the addition of 30  mol/L paraquat stimulated the activity of manganese dependent peroxidase (MnP), lignin peroxidase(LiP), and laccases (Lac) 7, 2.5 and 1.3 times, respectively. Also, addition of paraquat enhanced activity of superoxide dismutase (SOD) and catalase (CAT) in the first 48 h. Impact of paraquat on ligninolytic enzymes was significant than that on antioxidant enzyme. Addition of paraquat enhanced phenolic compounds and formaldehyde of cultures too. And concentration of malondialdehyde was increased in the first 24 h. The results showed that addition of paraquat promoted oxidative stress, but the antioxidant systems of the fungal strain are sufficient to prevent mycelia from oxidative stress. As exogenous oxygen, paraquat might be a useful substrate in degradation of lignocellulose.

Keywords: Coriolus versicolor, paraquat, oxidative stress, ligninolytic enzymes, formaldehyde

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