微生物學(xué)通報 FEB 20,2010,37(2):234～238
Rhizobium sp. N613胞外多糖的抗氧化活性
陳艷麗 任盛 魏國琴 呂利華 趙良啟*
(山西大學(xué)化學(xué)生物學(xué)與分子工程教育部重點(diǎn)實(shí)驗(yàn)室 山西 太原 030006)
摘 要: 以鐵氰化鉀和鐵離子為檢測系統(tǒng)測定了Rhizobium sp. N613胞外多糖(REPS)對氧化物的還原力; H2O2和Fe2+為羥自由基生成系統(tǒng)檢測了REPS對羥自由基的清除作用; 鄰苯三酚自氧化法檢測了REPS對超氧陰離子的清除作用; 并體外檢測了REPS對H2O2引起的氧化溶血現(xiàn)象的抑制作用及對小鼠肝組織脂質(zhì)過氧化損傷的保護(hù)作用,。結(jié)果顯示, REPS對氧化物具有明顯的還原力, 其對羥自由基的清除作用達(dá)到35.46%(多糖濃度為2 mg/mL), 對超氧陰離子的清除作用達(dá)到36.84% (多糖濃度為0.78 mg/mL), 對H2O2引起的氧化溶血現(xiàn)象的抑制作用達(dá)到43.84% (多糖濃度為1.14 mg/mL), 對小鼠肝組織脂質(zhì)過氧化的保護(hù)作用達(dá)到34.46% (多糖濃度為1.14 mg/mL), 表明REPS具有良好的抗氧化作用, 有著重要的應(yīng)用價值,。
關(guān)鍵詞: 錦雞兒根瘤菌胞外多糖, 活性氧自由基, 脂質(zhì)過氧化, 抗氧化活性
Antioxidant Activity of Exopolysaccharide from Rhizobium sp. N613
CHEN Yan-Li REN Sheng WEI Guo-Qin LV Li-Hua ZHAO Liang-Qi*
(Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,
Shanxi University, Taiyuan, Shanxi 030006, China)
Abstract: We investigated the reducing capacity of Caragana rhizobium exopolysaccharides (REPS) by the system of potassium ferricyanide and ferric chloride, determined the hydroxyl radical scavenging capacity of REPS by H2O2/Fe2+ system, measured the Superoxide radical scavenging capacity of REPS by the pyrogallol system, and we also determined its protective effects on H2O2 in hemplysis of erythrocytes and lipid per-oxidation of liver homogenate in rat. The results indicated that REPS has reductive capacity to oxides, the hydroxyl radical scavenging capacity of REPS was 35.46% (at the concentration of 2 mg/mL), the Su-peroxide radical scavenging capacity of REPS was 36.84% (at the concentration of 0.78 mg/mL), the protec-tive effects of REPS on H2O2 induced hemolysis of rat erythrocytes was 43.84% (at the concentration of 1.14 mg/mL), and the protective effects on lipid per-oxidation of liver homogenate in rat was 34.46% (at the concentration of 1.14 mg/mL).
Keywords: Exopolysaccharide from Rhizobium sp. N613, Reactive oxygen species, Lipid per-oxidation, Antioxidant activity
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