9月26日，國際知名學術雜志Journal of Virology在線發(fā)表了關于流感病毒調(diào)控宿主細胞周期的研究論文,。這項研究成果由中科院上海巴斯德研究所孫兵研究員課題組完成。
在本研究中,，研究人員觀察了流感病毒對細胞周期的影響,。研究結果表明，流感病毒A/WSN/33 (H1N1)的復制可以使細胞周期阻滯在G0/G1期,。一些調(diào)控細胞周期由G0/G1期向S期轉(zhuǎn)換的蛋白如高度磷酸化的Rb,， p21，cyclinE,，cyclinD1等在病毒感染后明顯下調(diào),。更為重要的是，研究人員還發(fā)現(xiàn)流感病毒通過將細胞周期阻滯在G0/G1期,，從而對病毒蛋白表達和病毒大量產(chǎn)生有重要作用,。研究還發(fā)現(xiàn)一些其它流感病毒株也具有類似的作用。由此可見,，這種作用有可能是流感病毒干預宿主細胞周期的一種普遍性機制,。該發(fā)現(xiàn)為流感病毒致病機理和抗病毒藥物的研究提供了新的思路。
該研究得到了國家自然科學基金委,、中科院,、上海市科委、國家863重點項目及李嘉誠基金會等項目資助,。（生物谷Bioon.com）
生物谷推薦英文摘要：
Journal of Virology doi:10.1128/JVI.01316-10
Dendritic Cell Activation by Recombinant Hemagglutinin Proteins of H1N1 and H5N1 Influenza A Viruses
Wen-Chun Liu,1 Shih-Chang Lin,1 Yen-Ling Yu,2 Ching-Liang Chu,2* and Suh-Chin Wu1,2*
Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan,1 Vaccine Research and Development Center, National Health Research Institutes, Zhunan, Miaoli County 350, Taiwan2
Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic H1N1 (A/Texas/05/2009), and highly pathogenic avian influenza H5N1 (A/Thailand/KAN-1/2004) viruses on mouse myeloid dendritic cells (mDCs). The results reveal that tumor necrosis factor alpha (TNF-), interleukin-12 (IL-12) p70, and major histocompatibility complex class II (MHC-II) expression was increased in mDCs after treatment with recombinant HA proteins of H1N1 and H5N1. The specificity of recombinant HA treatments for mDC activation was diminished after proteinase K digestion. HA apparently promotes mDC maturation by enhancing CD40 and CD86 expression and suppressing endocytosis. No significant differences in mDC activation were observed among recombinant proteins of H1N1 and H5N1. The stimulation of mDCs by HA proteins of H1N1 and H5N1 was completely MyD88 dependent. These findings may provide useful information for the development of more-effective influenza vaccines.
