著名國(guó)際病毒學(xué)期刊Journal of Virology（84:12075）發(fā)表了中科院廣州生物醫(yī)藥與健康研究院的最新研究成果。該成果首次報(bào)告成功研制了穩(wěn)定攜帶報(bào)告基因的復(fù)制型甲型流感病毒,。
近年來(lái),，流感病毒的流行以及快速變異對(duì)人類健康提出了嚴(yán)峻的挑戰(zhàn),，因此流感病毒的致病機(jī)理亟待進(jìn)一步闡明，人類也迫切需要更為安全有效的新型流感疫苗及抗流感藥物,。廣州生物醫(yī)藥與健康研究院呼吸疾病國(guó)家重點(diǎn)實(shí)驗(yàn)室陳凌博士研究團(tuán)隊(duì),，通過(guò)對(duì)實(shí)驗(yàn)室用非致病株P(guān)R8流感病毒基因組NA片段進(jìn)行改造，整合入增強(qiáng)型綠色熒光蛋白報(bào)告基因（EGFP）,，同時(shí)保留了完整的流感病毒基因組的復(fù)制型甲型流感病毒,，克服了之前其他的報(bào)道均是復(fù)制缺陷型流感病毒所存在的問(wèn)題。攜帶報(bào)告基因的復(fù)制型甲型流感病毒可以在細(xì)胞以及雞胚中穩(wěn)定生長(zhǎng),、傳代并表達(dá)報(bào)告基因,，因此可以很好的的模擬野生型病毒；同時(shí),，報(bào)告基因的檢測(cè)便捷可靠,，可用于篩選與評(píng)價(jià)抗流感病毒藥物和抗體,。
更有意義的是，檢測(cè)報(bào)告基因的表達(dá)情況可以用來(lái)實(shí)時(shí)追蹤流感病毒在動(dòng)物模型體內(nèi)的感染,、生長(zhǎng)與分布,。因此，該成果不但為流感病毒的基礎(chǔ)研究,，也為流感疫苗,、抗流感病毒藥物和抗體的研發(fā)提供了強(qiáng)有力的工具。
此項(xiàng)研究得到了中國(guó)科學(xué)院,、國(guó)家自然科學(xué)基金,、呼吸疾病國(guó)家重點(diǎn)實(shí)驗(yàn)室、廣東省自然科學(xué)基金等資助,。（生物谷Bioon.com）
生物谷推薦原文出處：
Journal of Virology doi:10.1128/JVI.00046-10
Generation of Replication-Competent Recombinant Influenza A Viruses Carrying a Reporter Gene Harbored in the Neuraminidase Segment
Feng Li,1, Liqiang Feng,1, Weiqi Pan,1 Zhenyuan Dong,1 Chufang Li,1 Caijun Sun,1 and Ling Chen1,2*
State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530,1 Department of Medical Biotechnology, University of Science & Technology of China, Hefei 230026, China2
Replication-competent influenza viruses carrying reporter genes are of great use for basic research, screening of antiviral drugs, and neutralizing of antibodies. In this study, two recombinant influenza A viruses with a neuraminidase (NA) segment harboring enhanced green fluorescent protein (EGFP) in the background of A/PR/8/34 (PR8) were generated. The viral RNA (vRNA)-specific packaging signals for NA were largely retained for efficient packaging. An "autocleave" 2A peptide sequence, which was inserted at the N terminus or the COOH terminus of NA to link with EGFP, enabled NA and EGFP to be expressed monocistronically. Further analysis demonstrated that both viruses, named rPR8-EGFP+NA and rPR8-NA+EGPF, although with some characteristic differences in growth and EGFP expression, could replicate in noncomplementary cells and propagate to large quantities while maintaining genome stability after multiple passages in embryonated eggs. These replication-competent influenza viruses carrying reporter genes are a great addition to the tool set for developing antiviral therapeutics and vaccines and for in vivo studies of viral dissemination and pathogenicity.
