豬鏈球菌2型莢膜缺失株生物學(xué)特性及其免疫保護(hù)性
胡丹 王長(zhǎng)軍 潘秀珍 唐家琪*
(南京軍區(qū)軍事醫(yī)學(xué)研究所 江蘇 南京 210002)
摘 要: 對(duì)前期構(gòu)建的豬鏈球菌2型中國(guó)強(qiáng)致病株05ZYH33莢膜缺失株Δcps2B進(jìn)行相關(guān)生物學(xué)特性及免疫保護(hù)性研究,。通過比較野生株05ZYH33和莢膜缺失株Δcps2B生物學(xué)特性發(fā)現(xiàn), Δcps2B株顯微形態(tài)發(fā)生改變, 失去與2型莢膜特異抗血清發(fā)生凝集反應(yīng)的能力, 突變株更易被全血清除,對(duì)上皮細(xì)胞HEP-2的粘附能力增強(qiáng),。動(dòng)物保護(hù)性實(shí)驗(yàn)結(jié)果表明該莢膜缺失株Δcps2B具有良好的免疫保護(hù)作用。研究結(jié)果提示莢膜在細(xì)菌抵抗吞噬和細(xì)菌粘附過程中發(fā)揮重要作用,。
關(guān)鍵詞: 豬鏈球菌2型, 莢膜多糖, 生物學(xué)特性, 免疫保護(hù)
英文摘要：
Characterization of a nonencapsulated mutant of Streptococcus suis serotype 2 and evaluation of its protective abilities in a mouse model
HU Dan WANG Chang-Jun PAN Xiu-Zhen TANG Jia-Qi*
(Research Institute for Medicine of Nanjing Command, Nanjing, Jiangsu 210002, China)
Abstract: The biological function of a formerly constructed nonencapsuled mutant Δcps2B from 05ZYH33 was characterized, and its protection against infection was evaluated. We found that Δcps2B have reduced ability to form long chain capsule polysaccharide and no longer agglutinated with the specific anti-capsular serum. The Δcps2B could be cleared more easily by the whole blood of human and piglet, while the capacity of adhesion to epithelial cells increased greatly. Animal infection experi-ment demonstrated that the Δcps2B protect mice from lethal challenge with S. suis 2. These observa-tions indicate that the capsule plays an essential role in the pathogenesis and invasiveness of S. suis 2.
Keywords: Streptococcus suis serotype 2, Capsular polysaccharide, Biological characteristics, Im-munoprotection
