據(jù)英國(guó)《每日郵報(bào)》5月2日?qǐng)?bào)道，美國(guó)華盛頓州立大學(xué)的科學(xué)家發(fā)現(xiàn),，大蒜中含有的一種關(guān)鍵成分在抗食物中毒方面，效力比抗生素高100倍,。
研究稱,，檢驗(yàn)發(fā)現(xiàn)大蒜中的烯丙基硫成分可以輕易破壞細(xì)菌的黏稠且具保護(hù)性的生物膜，這層生物膜可保護(hù)細(xì)菌不被毀滅,。此外,，烯丙基硫不僅比紅霉素和環(huán)丙沙星這兩種抗生素的效力更強(qiáng)大,，且見效更快。這項(xiàng)發(fā)現(xiàn)可能為找出處理生肉,、加工肉的方法找到新路,，從而降低感染彎曲桿菌導(dǎo)致的食物中毒危險(xiǎn)。
華盛頓州立大學(xué)研究人員邁克爾·康克爾（Michael Konkel）說：“這項(xiàng)研究成果令人激動(dòng),，因?yàn)檠芯匡@示,，烯丙基硫也許可減少環(huán)境以及食物供應(yīng)中的病菌。彎曲桿菌是美國(guó)乃至全世界引發(fā)食物傳染性疾病的最常見病菌,。”其感染癥狀包括腹瀉,、痙攣、腹痛和發(fā)燒等,。（生物谷Bioon.com）
doi: 10.1093/jac/dks138
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Antimicrobial effect of diallyl sulphide on Campylobacter jejuni biofilms
Xiaonan Lu1,2,3, Derrick R. Samuelson1, Barbara A. Rasco2 and Michael E. Konkel1,*
Objectives Bacterial biofilms pose significant food safety risks because of their attachment to fomites and food surfaces, including fresh produce surfaces. The purpose of this study was to systematically investigate the activity of selected antimicrobials on Campylobacter jejuni biofilms.
Methods C. jejuni biofilms and planktonic cells were treated with ciprofloxacin, erythromycin and diallyl sulphide and examined using infrared and Raman spectroscopies coupled with imaging analysis.
Results Diallyl sulphide eliminated planktonic cells and sessile cells in biofilms at a concentration that was at least 100-fold less than used for either ciprofloxacin or erythromycin on the basis of molarity. Distinct cell lysis was observed in diallyl sulphide-treated planktonic cells using immunoblot analysis and was confirmed by a rapid decrease in cellular ATP. Two phases of C. jejuni biofilm recalcitrance modes against ciprofloxacin and erythromycin were validated using vibrational spectroscopies: (i) an initial hindered adsorption into biofilm extracellular polymeric substance (EPS) and delivery of antibiotics to sessile cells within biofilms; and (ii) a different interaction between sessile cells in a biofilm compared with their planktonic counterparts. Diallyl sulphide destroyed the EPS structure of the C. jejuni biofilm, after which the sessile cells were killed in a similar manner as planktonic cells. Spectroscopic models can predict the survival of sessile cells within biofilms.
Conclusions Diallyl sulphide elicits strong antimicrobial activity against planktonic and sessile C. jejuni and may have applications for reducing the prevalence of this microbe in foods, biofilm reduction and, potentially, as an alternative chemotherapeutic agent for multidrug-resistant bacterial strains.
