生物谷報(bào)道：干細(xì)胞和祖細(xì)胞定向分化是目前研究熱點(diǎn)之一，而結(jié)合三維（立體）材料進(jìn)行定向分化，則被認(rèn)為是組織工程上最有希望的前景,。過去多采用高分子可降解的多聚物,，同時(shí)附上不同的藥物,，使胚胎干細(xì)胞能定向分化,，且能形成三維結(jié)構(gòu)，這一成果發(fā)表在去年10月分的PNAS上(見生物谷內(nèi)相關(guān)報(bào)道)。而這一自主性體溫調(diào)節(jié)研究成果則是將納米纖維上附接上兩性肽分子,從而使神經(jīng)祖細(xì)胞選擇性分化,這一研究成果開創(chuàng)了材料與定向分化結(jié)合的新篇章,同時(shí)也為組織工程開辟了新的領(lǐng)域和方向,。
Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers
Gabriel A. Silva,1* Catherine Czeisler,2* Krista L. Niece,3 Elia Beniash,3 Daniel A. Harrington,3 John A. Kessler,2 Samuel I. Stupp1,3,4
Science, Vol. 303, Issue 5662, 1352-1355, February 27, 2004
Neural progenitor cells were encapsulated in vitro within a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile molecules. The self-assembly is triggered by mixing cell suspensions in media with dilute aqueous solutions of the molecules, and cells survive the growth of the nanofibers around them. These nanofibers were designed to present to cells the neurite-promoting laminin epitope IKVAV at nearly van der Waals density. Relative to laminin or soluble peptide, the artificial nanofiber scaffold induced very rapid differentiation of cells into neurons, while discouraging the development of astrocytes. This rapid selective differentiation is linked to the amplification of bioactive epitope presentation to cells by the nanofibers.
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