山東省干細胞工程技術(shù)研究中心主任,、煙臺毓璜頂醫(yī)院中心實驗室對媒體宣布,，中國科學(xué)家已成功獲得人類體細胞克隆胚胎。
山東省干細胞工程技術(shù)研究中心利用不同來源的人類成體細胞成功克隆出五枚符合國際公認技術(shù)鑒定指標的人類囊胚,。該項研究成果已經(jīng)發(fā)表在今年一月二十七日出版的克隆和干細胞領(lǐng)域國際權(quán)威學(xué)術(shù)期刊CLONING AND STEM CELLS上,。
研究主要是采用了先進的三維立體偏震光紡錘體成像系統(tǒng)，對卵母細胞紡錘體（核DNA）精確定位后,，再用微激光對卵子的透明帶打孔,，精確剔除卵子細胞核。通過核移植后所獲得的囊胚進行了核的DNA遺傳多態(tài)性位點鑒定,，不同細胞階段克隆胚胎的供體與受體細胞漿中線粒體定量動態(tài)學(xué)分析和囊胚線粒體遺傳多態(tài)性位點SNP鑒定,。不但應(yīng)用人類成纖維體細胞獲得克隆胚胎，更重要的是應(yīng)用帕金森病患者外周血的淋巴細胞作為供體細胞也成功獲得囊胚，這使人類治療性克隆研究向前邁進了一大步,。（生物谷Bioon.com）
生物谷推薦原始出處：
Cloning and Stem Cells. ahead of print. doi:10.1089/clo.2008.0041.
Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach
Jianyuan Li,Xuexia Liu,Haiyan Wang,Shouxin Zhang,Fujun Liu,Xuebo Wang,Yanwei Wang
Shandong Research Center of Stem Cell Engineering, China.
Somatic cell nuclear transfer (SCNT) was used to generate patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs). In this study, a total of 135 oocytes were obtained from 12 healthy donors (30–35 years). Human oocytes, obtained within 2 h following transvaginal aspiration, were enucleated using a Spindle Imaging System to position the spindle and chromosomes that lay on the metaphase plate, and a Zona Infrared Laser Optical System was used to open a single hole in the zona pellucida at the 2 o'clock position. Human fibroblasts and lymphocytes were used to construct SCNT embryos. Nearly half (26 of 58) of the oocytes were fused after electrofusion and embryo development rates were 96.2% (two-cell, 25 of 26), 92.3% (four-cell, 24 of 26), 61.5% (eight-cell, 16 of 26), 34.6% (16-cell, 9 of 26), 26.9% (morula, 7 of 26), and 19.2% (blastocyst, 5 of 26), respectively, following incubation in improved G-series sequential medium. One cloned blastocyst was used for STR-DNA identification and genetic polymorphism analysis of mtDNA, and STR-DNA analysis of all cloned blastocysts indicated they were derived from SCNT. Quantitative analysis showed that mtDNA of cloned embryos reflected the change tendency of those observed in human IVF embryos. Our research provides an alternative enucleation approach for producing human SCNT-derived blastocysts, and may aid in providing a new method for human therapeutic cloning.
