山東省干細(xì)胞工程技術(shù)研究中心主任、煙臺(tái)毓璜頂醫(yī)院中心實(shí)驗(yàn)室對(duì)媒體宣布,，中國(guó)科學(xué)家已成功獲得人類(lèi)體細(xì)胞克隆胚胎,。
山東省干細(xì)胞工程技術(shù)研究中心利用不同來(lái)源的人類(lèi)成體細(xì)胞成功克隆出五枚符合國(guó)際公認(rèn)技術(shù)鑒定指標(biāo)的人類(lèi)囊胚,。該項(xiàng)研究成果已經(jīng)發(fā)表在今年一月二十七日出版的克隆和干細(xì)胞領(lǐng)域國(guó)際權(quán)威學(xué)術(shù)期刊CLONING AND STEM CELLS上,。
研究主要是采用了先進(jìn)的三維立體偏震光紡錘體成像系統(tǒng)，對(duì)卵母細(xì)胞紡錘體（核DNA）精確定位后,，再用微激光對(duì)卵子的透明帶打孔,，精確剔除卵子細(xì)胞核。通過(guò)核移植后所獲得的囊胚進(jìn)行了核的DNA遺傳多態(tài)性位點(diǎn)鑒定,，不同細(xì)胞階段克隆胚胎的供體與受體細(xì)胞漿中線粒體定量動(dòng)態(tài)學(xué)分析和囊胚線粒體遺傳多態(tài)性位點(diǎn)SNP鑒定,。不但應(yīng)用人類(lèi)成纖維體細(xì)胞獲得克隆胚胎，更重要的是應(yīng)用帕金森病患者外周血的淋巴細(xì)胞作為供體細(xì)胞也成功獲得囊胚,，這使人類(lèi)治療性克隆研究向前邁進(jìn)了一大步,。（生物谷Bioon.com）
生物谷推薦原始出處：
Cloning and Stem Cells. ahead of print. doi:10.1089/clo.2008.0041.
Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach
Jianyuan Li,Xuexia Liu,Haiyan Wang,Shouxin Zhang,Fujun Liu,Xuebo Wang,Yanwei Wang
Shandong Research Center of Stem Cell Engineering, China.
Somatic cell nuclear transfer (SCNT) was used to generate patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs). In this study, a total of 135 oocytes were obtained from 12 healthy donors (30–35 years). Human oocytes, obtained within 2 h following transvaginal aspiration, were enucleated using a Spindle Imaging System to position the spindle and chromosomes that lay on the metaphase plate, and a Zona Infrared Laser Optical System was used to open a single hole in the zona pellucida at the 2 o'clock position. Human fibroblasts and lymphocytes were used to construct SCNT embryos. Nearly half (26 of 58) of the oocytes were fused after electrofusion and embryo development rates were 96.2% (two-cell, 25 of 26), 92.3% (four-cell, 24 of 26), 61.5% (eight-cell, 16 of 26), 34.6% (16-cell, 9 of 26), 26.9% (morula, 7 of 26), and 19.2% (blastocyst, 5 of 26), respectively, following incubation in improved G-series sequential medium. One cloned blastocyst was used for STR-DNA identification and genetic polymorphism analysis of mtDNA, and STR-DNA analysis of all cloned blastocysts indicated they were derived from SCNT. Quantitative analysis showed that mtDNA of cloned embryos reflected the change tendency of those observed in human IVF embryos. Our research provides an alternative enucleation approach for producing human SCNT-derived blastocysts, and may aid in providing a new method for human therapeutic cloning.
