科學(xué)家開(kāi)發(fā)出了一種大鼠心臟的“補(bǔ)丁”，它可以在活組織內(nèi)部培養(yǎng),，然后移植到宿主動(dòng)物的心臟中從而修復(fù)肌肉組織。用新的細(xì)胞修補(bǔ)心臟可以有助于該器官的復(fù)原并增強(qiáng)其功能——心臟在經(jīng)歷了心臟病發(fā)作的損傷之后無(wú)法讓細(xì)胞再生,。
Smadar Cohen 及其同事開(kāi)發(fā)出了一種把在實(shí)驗(yàn)室中生長(zhǎng)的細(xì)胞轉(zhuǎn)移到受傷的心臟中的方法,，該方法還包括利用身體作為生物反應(yīng)器的一個(gè)中間步驟。這組科學(xué)家用新生大鼠心臟細(xì)胞和一組生長(zhǎng)因子構(gòu)成了基本的框架,。在48小時(shí)后,，他們把這個(gè)心臟補(bǔ)丁移植到了大鼠腹部?jī)?nèi)部。在7天的時(shí)間里,，浸在生長(zhǎng)因子中的這個(gè)補(bǔ)丁在腹部網(wǎng)膜的血管豐富的組織上培養(yǎng),。這一中間過(guò)程可以讓這個(gè)補(bǔ)丁發(fā)育出存活和產(chǎn)生功能所需的毛細(xì)血管網(wǎng)和肌纖維。一旦植入心臟,，這個(gè)補(bǔ)丁就繼續(xù)發(fā)育,，并與大鼠心臟組織結(jié)合起來(lái)。這組作者說(shuō),，在28天后,，與對(duì)照組相比，這些實(shí)驗(yàn)動(dòng)物在經(jīng)過(guò)修補(bǔ)的心臟區(qū)域出現(xiàn)了更廣泛的血管形成,，而且修復(fù)的組織把它的細(xì)胞沖動(dòng)節(jié)律與原來(lái)的臨近細(xì)胞的節(jié)律連接了起來(lái),。（生物谷Bioon.com）
生物谷推薦原始出處：
PNAS August 24, 2009, doi: 10.1073/pnas.0812242106
Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
Tal Dvira, Alon Kedemb, Emil Ruvinova, Oren Levyc, Inbar Freemana, Natalie Landad, Radka Holbovad, Micha S. Feinbergd, Shani Drore, Yoram Etzione, Jonathan Leord and Smadar Cohena,1
aDepartment of Biotechnology Engineering and
cDepartment of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel 84105;
bDepartment of Obstetrics and Gynecology and
dNeufeld Cardiac Research Institute, Sheba Medical Center, Tel-Aviv University, Tel-Hashomer, Israel 52621; and
eCardiac Arrhythmia Research Laboratory, Soroka University Medical Center, Beer-Sheva, Israel 84105
The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.
